Applications Progress of 3D Culture of Stem Cells in the Research of Dental Pulp Regeneration

doi:10.13701/j.cnki.kqyxyj.2021.11.001

Abstract

Abstract: 3D (Three-dimensional) culture of cells is a culture method in vitro. The growth and original 3D shape of cells in human body are simulated by this method which is beneficial to intracellular gene expression and signal transduction. As one of the important research directions, 3D culture of stem cells was mainly used in the field of organ culture and tissue regeneration. In the study of dental pulp regeneration, there are some limitations of cell contact inhibition, gene expression, and loss of biological functions in 2D (two-dimensional) culture of stem cells. However, the ability of self-renewal and specific differentiation of stem cells are enhanced by 3D culture and the regeneration effect of pulp-dentin complex with neurovascularization is significantly better than 2D monolayer culture. Therefore, in this review, the research status of 3D culture of cells are discussed. The advantage, application and prospect of 3D culture of stem cells in the research of dental pulp regeneration are summarized and analyzed. It is aimed to provide a theoretical basis for the optimization of culture conditions of stem cells and regeneration effect in dental pulp regeneration.

Key words: 3D culture, pulp, dental pulp regeneration, stem cells

NIU Weidong, LIU Runyuan. Applications Progress of 3D Culture of Stem Cells in the Research of Dental Pulp Regeneration[J]. Journal of Oral Science Research, 2021, 37(11): 961-965.

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