Effects of Low Intensity Pulsed Ultrasound on Hippo Signaling Pathway in Human Periodontal Membrane Cells

doi:10.13701/j.cnki.kqyxyj.2022.11.017

Abstract

Abstract: Objective: To observe the effects of low intensity pulsed ultrasound (LIPUS) on the genes expression of Hippo signaling pathway in human periodontal ligament cells (hPDLCs). Methods: The primary cells were cultured, and the third generation of periodontal cells were stimulated with LIPUS for 3 days with the intensity of 30 mW/cm², 45 mW/cm², and 60 mW/cm² for 25 min. Western blot and Q-PCR were used to detect the genes expression of Hippo signaling pathway. The changes of cell morphology were observed, and the optimal treatment conditions were screened. Alizarin red staining was performed on cells stimulated for 21 days to analyze the effect of LIPUS on osteogenesis compared with the control group. Results: In this study, primary periodontal membrane cells were successfully cultured. The expression of YAP, TAZ, and RUNX2 in Hippo signaling pathway was significantly enhanced, and the p-TAZ and p-YAP expressions were down-regulated in periodontal membrane cells with LIPUS stimulated. The osteogenic effect of 45 mW/cm² and 60 mW/cm² was more obvious than that of 30 mW/cm². In contrast to the control group, calcium nodules increased significantly after LIPUS (60 mW/cm²) stimulation. Conclusion: Low-pulse ultrasound with appropriate intensity can promote the expression of osteogenic genes of Hippo signaling pathway in periodontal membrane cells, which can provide a theoretical basis to optimize the orthodontic environment.

Key words: dental orthodontics, low intensity pulsed ultrasound, human periodontal ligament cells, Hippo signaling pathway

LI Ping, YIN Juan, XU Minxin, XUE Hui. Effects of Low Intensity Pulsed Ultrasound on Hippo Signaling Pathway in Human Periodontal Membrane Cells[J]. Journal of Oral Science Research, 2022, 38(11): 1087-1091.

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