Evaluation of FTA-DNA Direct Extraction Method in the Detection of Peri-implantitis or Periodontitis.

Abstract

Abstract: Objective: To evaluate the effect of FTA-DNA direct extraction method in the detection of peri-implantitis (PI) and periodontitis. Methods: Fourteen PI patients and 26 periodontitis patients were selected. Traditional method, boiling method, and FTA-DNA direct extraction method were used respectively to extract DNA. 16S-rDNA was amplified and gel electrophoresis was carried to compare the differences among three DNA extraction methods. Results: There was no significant difference between the peri-implantitis and periodontitis. DNA could be extracted by these three methods. FTA-DNA direct extraction significantly increased the success rate, when compared with traditional method and boiling method. Conclusion: Bacterial DNA in PI and periodontitis patients can be efficiently extracted and saved by FTA-DNA direct extraction method, which may be used to study and diagnose peri-implantitis and periodontitis.

CLC Number:

R781.4

WU Ya-ting, NIE Hong-bing, WANG Zi-jian, WANG Jia-jia, ZHOU Jian-ye, GUO Man-li.. Evaluation of FTA-DNA Direct Extraction Method in the Detection of Peri-implantitis or Periodontitis.[J]. Journal of Oral Science Research, 2015, 31(11): 1089-1092.

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