Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation.

doi:10.13701/j.cnki.kqyxyj.2016.03.010

Abstract

Abstract: Objective: To investigate osteoblast adhesion, growth and proliferation on the alendronate sAium (ALN) and collagen type I (COL I) coated nano-hydroxyapatite (nHA) surface. Methods: nHA layer, nHA/ALN complex film, nHA/COL I complex film, nHA/ALN/COL I complex film, and nHA/COL I/ALN complex film were established. Osteoblasts were cultured on the films for 7 days and 14 days. The morphology of osteoblast was observed by SEM and the cell proliferation was determined by WST-1 assay, respectively. Results: The results revealed that nHA/ALN/COL I and nHA/COL I/ALN complex films accelerated osteoblasts growth and proliferation rate (P<0.05). The osteoblasts showed fully extended morphology combined with numerous pseudopodia and microvilli structure by directly contact to the collagen layer. Conclusion: ALN and COL I exhibited synergistic effect in accelerating adhesion and proliferation of osteoblasts in vitro. The nHA/ALN/COL I complex film benefited osteoblast adhesion and differentiation.

Key words: Nano-hydroxyapatite, Alendronate sAium , Collagen type I , Osteoblast

CLC Number:

R783.1

JIANG Yan, YAO Jiang-Wu. Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation.[J]. Journal of Oral Science Research, 2016, 32(3): 253-256.

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