Abstract: Objective: To observe the repair of mandibular defects with tissue engineering bone using coral hydroxyapatite and dog marrow stromal cells (dMSCs) which was transfected by recombinant adenovirus vector in vitro carrying human bone morphogenetic protein-7 (hBMP-7) gene. Methods: After the compound of coral hydroxyapatite composite scaffold material and dMSCs transfected with high-level expression hBMP-7 adenovirus vector constructed by AdEasy adenovirus expression system was implanted in mandibular defects of dogs, the reparation situation of mandibular defection was observed 4 weeks and 8 weeks after operation respectively. Results: Significant new bone formation was found in the naked mouse skin and bone defect by gross observation, X-ray examination and histological observation of the constructed tissue engineering bone. Conclusion: Constructed tissue engineering bone carrying hBMP-7 gene was found to format more volume bone tissue, and could effectively repair mandibular defect.

Key words: Human bone morphogenetic protein 7, Marrow stromal cells, Adenovirus , Tissue engineering