Cytocompatibility Study of Silk Fibroin/poly-L-lactic Acid Scaffold and Chondrocytes in Vitro.

Abstract

Abstract: Objective: To lay the foundation for further body and animal experiment, we observe the cytocompatibility on electrostatic spinning scaffold of silk fibroin/poly-L-lactic acid (SF/PLLA) in vitro and explore the feasibility in cartilage tissue engineering scaffolds. Methods: The rabbit knee joint chondrocyte were co-cultured with silk fibroin/poly-L-lactic acid (SF/PLLA) scaffold. Hematoxylin-eosin staining and Alcian blue- nuclear fast red staining were tested on day 3, 7 and 14 respectively. Cell adhesion was tested by scanning electron microscopy (SEM). Cell proliferation was determined by MTT test. Results: Adhesion, proliferation, and growth of chondrocyte derived from rabbit knee joint chondrocytes were great on the surface of silk fibroin/poly-L-lactic acid (SF/PLLA). The chondrocytes cultured in vitro maintained the specific chondrocytes phenotype on the scaffold. Conclusion: Silk fibroin/poly-L-lactic acid (SF/PLLA) scaffold exhibits excellent cytocompatibility, and can be used for cartilage tissue engineering.

Key words: Silk fibroin/poly-L-lactic acid (SF/PLLA), Tissue engineering, Cartilage

CLC Number:

R782.2

SUN Ying, LI Zheng-qiang, LIU Wei-wei, et al.. Cytocompatibility Study of Silk Fibroin/poly-L-lactic Acid Scaffold and Chondrocytes in Vitro.[J]. Journal of Oral Science Research, 2015, 31(3): 221-225.

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