Observation of GFP-labeled 3D-printed Jaw using Laser Confocal Microscopy in Vivo.

doi:10.13701/j.cnki.kqyxyj.2016.10.005

Abstract

Abstract: Objective: To observe GFP-labeled 3D-printed jaw by using laser confocal microscopy in vivo and to provide technical basis for the follow-up function study. Methods: The calcined ceramic powder was made from sheep vertebrae, which was combined with polyvinyl alcohol to form jaw scaffold. Sheep BMSCs were infected by GFP lentivirus and cultured on the scaffold. The scaffold was implanted into sheep jaw defect and observed by confocal laser scanning microscope at different time points. Results: The infection efficiency was about 95.4% detected by flow cytometry. BMSCs could survive for more than 3 months on the scaffold determined by three-dimensional imaging technique using laser scanning confocal microscopy after implantation. Conclusion: Laser scanning confocal microscopy can be used to observe the GFP-labeled 3D-printed jaw.

Key words: GFP, Lentivirus, Tissue engineering, Confocal laser scanning microscope

CLC Number:

R783.2

HAN Xiang-zhen, ZHOU Qi-qi, HU Yang, SONG Yan-yan, HE Hui-yu.. Observation of GFP-labeled 3D-printed Jaw using Laser Confocal Microscopy in Vivo.[J]. Journal of Oral Science Research, 2016, 32(10): 1020-1023.

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