Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice

doi:10.13701/j.cnki.kqyxyj.2016.11.002

Abstract

Abstract: Objective: To detect the expressions of Amelogenin and Ameloblastin in wild-type mice and MMP-9 gene knock-out mice (MMP-9^-/-). Methods: The expression levels of Amelogenin and Ameloblastin in tooth germs were detected with immunohistochemistry in wild-type and MMP-9^-/- mice at post-natal day 2 (P2), 5 (P5), and 7 (P7). Results: At P2, Amelogenin was mainly expressed in matured ameloblasts and the underlying odontoblasts. However, in MMP-9^-/- mice, the expression of amelogenin was expanded to the dental mesenchyme. No difference was found in the expression pattern of Amelogenin between wild-type mice and MMP-9^-/- mice at P5 and P7. However, the expression level of Amelogenin in ameloblasts was highly increased in MMP-9^-/- mice compared with that in wild-type mice. No obvious differences were detected in the expression pattern or expression level of Ameloblastin between wild-type mice and MMP-9^-/- mice at P2, P5, and P7. Conclusion: MMP-9 might be able to degrade Amelogenin, and further influence the expression pattern of Amelogenin.

Key words: Amelogenin, Ameloblastin, Molar germ, Matrix, Metalloproteinase-9

CLC Number:

Q132

LI Tong, YUAN Guo-hua, NIE Min, YANG Guo-bin. Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice[J]. Journal of Oral Science Research, 2016, 32(11): 1117-1121.

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