Utilization of Bacterial Array-CGH to Compare the Differences of Genomic in Different Strains of Porphyromonas Gingivalis.

doi:10.13701/j.cnki.kqyxyj.2016.05.020

Abstract

Abstract: Objective: To identify the genomic differences among different strains of Porphyromonas gingivalis(P.gingivalis)to provide a basis for further exploration of periodontitis. Methods: According to the whole-genome sequencing of 12 P.gingivalis strains, the probes and the whole-genomic chip were designed. After extracting the genomic DNA of highly toxic strain P.gingivalis W83 and minimally toxic strain P.gingivalis ATCC 33277, array-CGH was used to compare the differences and PCR was employed to verify the array results and accuracy. Results: This global comparative analysis showed the difference of continuous fragments between W83 and ATCC 33277, and the unique genes in W83 might contribute to the virulence and associate with the invasion ability of P.gingivalis. Verification with PCR confirmed the consistency with array-CGH. Conclusion: Array-CGH is an effective method to compare the genomic differences among clinical strains of periodontitis.

CLC Number:

R781.4

LI Xin-yue, ZHANG Liang, LV Li-juan, MA Jian, ZHOU Wei-ping, HUANG Shu-jun, ZHOU Xiang-cheng, HE Jun-cheng, YE Ning.. Utilization of Bacterial Array-CGH to Compare the Differences of Genomic in Different Strains of Porphyromonas Gingivalis.[J]. Journal of Oral Science Research, 2016, 32(5): 506-509.

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