Abstract: Objective: To study the effect of basic fibroblast growth factors (bFGF, referred as B) and Mibefradil (referred as M) on proliferation of human periodontal ligament fibroblast (HPLFs). Methods: B with three concentrations of 10, 20 and 30ng/mL were respectively defined as B1, B2 and B3; M with three concentrations of 2.5, 5 and 10μmol/L were respectively defined as M1, M2 and M3; and B and M with three concentrations were respectively sub-divided into a 1-day group, a 2-day group and a 3-day group. The WST method was employed to detect the proliferation rate treated with one drug alone or combined usage of the two drugs in different orders. Results:B increased the proliferation of HPLFs, while M was on the contrary. The B3 1-day group showed the highest cell proliferation rate (P<0.05), and the M2 1-day group showed the lowest cell proliferation. There was significant difference in proliferation rate in B3 group combined with or without M (P<0.05). In addition, only B1 could not change the proliferation rate inhibited by the addition of M. There was no statistical difference in proliferation rate between combined usage groups and normal cells groups (P>0.05). Conclusion: bFGF and Mibefradil have their respective optimum acting concentration and acting time; Except the excessive proliferation state, the proliferation state of HPLFs is hard to block, and the blocking cells are easy to restore their proliferation state to continuously maintain the HPLFs in a balanced proliferation state.

Key words: Human periodontal ligament fibroblasts, Proliferation bFGF, Mibefradil