Relationship between Lymph Node Metastasis and Long Non-coding RNA HOTAIR in Tongue Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2017.02.024

Abstract

Abstract: Objective: To investigate the correlation between Hox transcript anti-sense intergenic RNA (HOTAIR) and lymph node metastasis in tongue squamous cell carcinoma (TSCC). Methods: The expression of HOTAIR was detected in 19 TSCC tissues and 19 matched nonmalignant tissues by using Real-time quantitative PCR, and its correlation with TNM stage was analyzed. The migration and invasion abilities were evaluated in CAL27 and SCC9 cell lines after silencing the expression of HOTAIR. Results: 26.3% of TSCC tissues showed a higher expression of HOTAIR compared to the matched nonmalignant tissues (P=0.0169), and all these patients underwent lymphatic metastasis. Patients without lymphatic metastasis showed a low expression of HOTAIR (P<0.001). After silencing the expression of HOTAIR, the tumor cells' ability of migration and invasion was decreased (P<0.001). Conclusion: High expression of HOTAIR may be an indicator of lymphatic metastasis in TSCC.

Key words: Tongue squamous cell carcinoma, Lymphatic metastasis , Long non-coding, RNA , HOTAIR

CLC Number:

R739.8

PIAO Zheng-guo, ZOU Rui, TANG Shan, OUYANG Ke-xiong. Relationship between Lymph Node Metastasis and Long Non-coding RNA HOTAIR in Tongue Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2017, 33(2): 216-220.

References

[1] Li T, Mo X, Fu L, et al. Molecular mechanisms of longnoncoding RNAs on gastric cancer [J]. Oncotarget, 2016, 23(8)∶8601-8612
[2] WU Y, ZHANG L, WANG Y, et al. Long noncoding RNA HOTAIR involvement in cancer [J].Tumour Biol, 2014, 35(10)∶9531-9538
[3] ZHANG S, CHEN S, YANG G, et al. Long noncoding RNA HOTAIR as an independent prognostic marker in cancer: a meta-analysis [J]. PLoS One, 2014, 9(8)∶e105538
[4] American Joint Committee on Cancer. AJCC Cancer Staging Manual [M]. 7th Edition (2010). AJCC. 2009∶1-4
[5] 欧阳可雄,梁军,邹瑞,等.舌鳞癌组织长链非编码RNA的Ion Torrent高通量检测和分析[J]. 口腔疾病防治,2016,24(1)∶15-19
[6] ZENG XH, OU ZL, YU KD, et al. Absence of multiple atypical chemokine binders (ACBs) and the presence of VEGF and MMP-9 predict axillary lymph node metastasis in early breast carcinomas [J]. Med Oncol, 2014, 31(9)∶145-152
[7] KOGO R, SHIMAMURA T, MIMORI K, et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers [J]. Cancer Res, 2011, 71(20)∶6320-6326
[8] ISHIBASHI M, KOGO R, SHIBATA K, et al. Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma [J]. Oncol Rep, 2013, 29(3)∶946-950
[9] NIINUMA T, SUZUKI H, NOJIMA M, et al. Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors [J]. Cancer Res, 2012, 72(5)∶1126-1136
[10] 付丛,张行炜.长链非编码RNA AFAP1-AS1在口腔鳞状细胞癌中的表达及临床意义[J].口腔医学研究,2016,32(9)∶983-986
[11] 邹瑞,林颖,欧阳可雄,等. H19基因在肿瘤发生发展中作用的研究新进展[J]..实用医学杂志,2015,31(19)∶151-153
[12] Dhamija S, Diederichs S. From junk to master regulators of invasion: lncRNA functions in migration, EMT and metastasis [J]. Int J Cancer, 2016, 15, 139(2)∶269-280
[13] Lee M, Kim HJ, Kim SW, et al. The long non-coding RNA HOTAIR increases tumour growth and invasion in cervical cancer by targeting the Notch pathway [J].Oncotarget, 2016, 15(1)∶29-32
[14] Lee NK, Lee JH, Kim WK, et al. Promoter methylation of PCDH10 by HOTAIR regulates the progression of gastrointestinal stromal tumors[J].Oncotarget. 2016, 21. [Epub ahead of print]
[15] Sun J, Chu H, Ji J, et al. Long non-coding RNA HOTAIR modulates HLA-G expression by absorbing miR-148a in human cervical cancer[J].Int J Oncol, 2016, 49(3)∶943-952