Immunophontyping of Peripheral Blood Lymphocytes in Patients with Oral Squamous Cell Carcinoma and Its Clinical Significance.

doi:10.13701/j.cnki.kqyxyj.2018.05.021

Abstract

Abstract: Objective: To investigate the distribution of immunophenotypes of peripheral blood lymphocytes in patients with oral squamous cell carcinoma (OSCC) and to explore its role in the tumor progression and its relationship with cancer-associated risk factors. Methods: Forty-nine patients with OSCC and 15 normal adults were enrolled in this study. Peripheral T and B lymphocytes and their immunophenotype distributions were detected by flow cytometry. The relevance of this distribution was analyzed with cancer-associated risk factors, including betel-chewing, smoking, alcohol drinking, tumor TNM stage, and lymph node metastasis. Results: The frequency of CD8⁺ T cells, effector memory T cells (CD4⁺Teff CD8⁺Teff), CD4⁺PD1⁺T, CD8⁺PD1⁺T, CD8⁺PD1⁺Teff, CD8⁺Tim3⁺T, and CD8⁺Tim3⁺Teff in OSCC was significantly higher than that of normal control group, whereas the CD4⁺ Naive T cells (Tn) and B cells were significantly decreased, and CD4⁺Tn/CD4⁺ Teff and CD8⁺Tn/CD8⁺Teff ratios were significantly decreased. The decrease of CD4⁺ Teff frequency was significantly associated with betel nut chewing, but the expression of CD4⁺ Tn cells was significantly increased (P<0.05). The history of drinking and smoking did not show any significantly difference in T and B cell immune subtypes. Conclusion: In this study, there were significant changes in the immunophenotypes of peripheral blood lymphocytes in patients with OSCC. The changes were significantly associated with betel chewing and tumor progression.

Key words: Oral squamous cell carcinoma, Peripheral blood lymphocytes, Lymph node metastasis, Tumor progression, Cancer-associated risk factors

YANG Liu, CHEN Juan, SHAN Zhong-yan, LEI Zhuo, QUAN Hong-zhi, TANG Zhan-gui. Immunophontyping of Peripheral Blood Lymphocytes in Patients with Oral Squamous Cell Carcinoma and Its Clinical Significance.[J]. Journal of Oral Science Research, 2018, 34(5): 548-553.

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