Effects of Mechanical Stimulation on Biological Behavior of Periodontal Ligament Stem Cells

doi:10.13701/j.cnki.kqyxyj.2023.05.004

Abstract

Abstract: Periodontal ligament stem cells (PDLSCs) have great potential in the treatment of periodontal diseases and other tissue regeneration fields, and the study of their biological behavior is helpful to the development of regenerative medicine. Mechanical stimuli such as mechanical stress, matrix surface morphology, and elasticity not only affect the proliferation and migration of PDLSCs, but also have the potential to induce the differentiation of PDLSCs into bone, tendon, myocardium, cornea, and other somatic cells, and this process is regulated by the time dimension. In addition, the perception of PDLSCs to mechanical stimuli also has memory. PDLSCs can retain the mechanical information in the past culture environment and affect the direction of their future differentiation. Current studies have confirmed that mechanical stimulation signals of PDLSCs can be transmitted into cells through integrins and ion channels to regulate cell behavior. In this paper, the effects of mechanical stimulation on the biological behavior of PDLSCs are reviewed, in order to provide new ideas for the regulation of PDLSCs differentiation in tissue engineering.

Key words: periodontal ligament stem cells, mechanical stimulation, cell differentiation, integrin, piezo protein

WANG Xiaoli, AN Xiaoli. Effects of Mechanical Stimulation on Biological Behavior of Periodontal Ligament Stem Cells[J]. Journal of Oral Science Research, 2023, 39(5): 396-401.

References

[1] Tomokiyo A, Yoshida S, Hamano S, et al. Detection, characterization, and clinical application of mesenchymal stem cells in periodontal ligament tissue [J]. Stem Cells Int, 2018, 2018: 5450768.
[2] Pelaez D, Acosta Torres Z, Ng TK, et al. Cardiomyogenesis of periodontal ligament-derived stem cells by dynamic tensile strain [J]. Cell Tissue Res, 2017, 367(2): 229-241.
[3] Chen J, Zhang W, Backman LJ, et al. Mechanical stress potentiates the differentiation of periodontal ligament stem cells into keratocytes [J]. Br J Ophthalmol, 2018, 102(4): 562-569.
[4] Goetzke R, Sechi A, De Laporte L, et al. Why the impact of mechanical stimuli on stem cells remains a challenge [J]. Cell Mol Life Sci, 2018, 75(18): 3297-3312.
[5] Wang W, Li N, Wang M, et al. Analysis of ceRNA networks during mechanical tension-induced osteogenic differentiation of periodontal ligament stem cells [J]. Eur J Oral Sci, 2022, 130(5): e12891.
[6] Chen D, Kim S, Lee S, et al. The effect of mechanical vibration on osteogenesis of periodontal ligament stem cells [J]. J Endod, 2021, 47(11): 1767-1774.
[7] Wu J, Li Y, Fan X, et al. Analysis of gene expression profile of periodontal ligament cells subjected to cyclic compressive force [J]. DNA Cell Biol, 2011, 30(11): 865-873.
[8] Jin SS, He DQ, Wang Y, et al. Mechanical force modulates periodontal ligament stem cell characteristics during bone remodelling via TRPV4 [J]. Cell Prolif, 2020, 53(10): e12912.
[9] Panchamanon P, Pavasant P, Leethanakul C. Periostin plays role in force-induced stem cell potential by periodontal ligament stem cells [J]. Cell Biol Int, 2019, 43(5): 506-515.
[10] Sun XY, Zheng GQ, Li CY, et al. Long non-coding RNA Fer-1-like family member 4 suppresses hepatocellular carcinoma cell proliferation by regulating PTEN in vitro and in vivo [J]. Mol Med Rep, 2019, 19(1): 685-692.
[11] Huang Y, Liu H, Guo R, et al. Long non-coding RNA FER1L4 mediates the autophagy of periodontal ligament stem cells under orthodontic compressive force via AKT/FOXO3 pathway [J]. Front Cell Dev Biol, 2021, 9: 631181.
[12] Zhang L, Liu W, Zhao J, et al. Mechanical stress regulates osteogenic differentiation and RANKL/OPG ratio in periodontal ligament stem cells by the Wnt/β-catenin pathway [J]. Biochim Biophys Acta, 2016, 1860(10): 2211-2219.
[13] Huang Y, Zhang Y, Li X, et al. The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force [J]. Eur J Orthod, 2019, 41(4): 333-342.
[14] 赵艳,刘佳,秦文,等.不同等级应力对人炎症牙周膜干细胞分化及细胞骨架重组的研究[J].临床口腔医学杂志,2019,35(3): 131-135.
[15] Lv P, Gao P, Tian G, et al. Osteocyte-derived exosomes induced by mechanical strain promote human periodontal ligament stem cell proliferation and osteogenic differentiation via the miR-181b-5p/PTEN/AKT signaling pathway [J]. Stem Cell Res Ther, 2020, 11(1): 1-15.
[16] Wang W, Li N, Zhao Y, et al. Effect of stretch frequency on osteogenesis of periodontium during periodontal ligament distraction [J]. Orthod Craniofac Res, 2023, 26(1):53-61.
[17] Suwittayarak R, Klincumhom N, Ngaokrajang U, et al. Shear stress enhances the paracrine-mediated immunoregulatory function of human periodontal ligament stem cells via the ERK signalling pathway [J]. Int J Mol Sci, 2022, 23(13): 7119.
[18] Zheng L, Shi Q, Na J, et al. Platelet-derived growth factor receptor-α and β are involved in fluid shear stress regulated cell migration in human periodontal ligament cells [J]. Cell Mol Bioeng, 2018, 12(1): 85-97.
[19] Li H, Deng Y, Tan M, et al. Low-intensity pulsed ultrasound upregulates osteogenesis under inflammatory conditions in periodontal ligament stem cells through unfolded protein response [J]. Stem Cell Res Ther, 2020, 11(1): 1-15.
[20] Li H, Zhou J, Zhu M, et al. Low-intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration [J]. J Biomed Mater Res A, 2021, 109(7): 1101-1112.
[21] Zhang C, Lu Y, Zhang L, et al. Influence of different intensities of vibration on proliferation and differentiation of human periodontal ligament stem cells [J]. Arch Med Sci, 2015, 11(3): 638-646.
[22] Yam GH, Teo EP, Setiawan M, et al. Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy [J]. J Cell Mol Med, 2018, 22(6): 3119-3132.
[23] Kilian KA, Bugarija B, Lahn BT, et al. Geometric cues for directing the differentiation of mesenchymal stem cells [J]. Proc Natl Acad Sci U S A, 2010, 107(11): 4872-4877.
[24] Hu P, Gao Q, Zheng H, et al. The role and activation mechanism of TAZ in hierarchical microgroove/nanopore topography-mediated regulation of stem cell differentiation [J]. Int J Nanomedicine, 2021, 16: 1021-1036.
[25] Kim J, Kang MS, Eltohamy M, et al. Dynamic mechanical and nanofibrous topological combinatory cues designed for periodontal ligament engineering [J]. PLoS One, 2020, 15(1): e0228475.
[26] Hegeds O, Juriga D, Sipos E, et al. Free thiol groups on poly(aspartamide) based hydrogels facilitate tooth-derived progenitor cell proliferation and differentiation [J]. PLoS One, 2019, 14(12):e0226363.
[27] Yan XZ, van den Beucken JJP, Yuan C, et al. Evaluation of polydimethylsiloxane-based substrates for in vitro culture of human periodontal ligament cells [J]. J Biomed Mater Res A, 2019, 107(12): 2796-2805.
[28] He C, Wang T, Wang Y, et al. ILK regulates osteogenic differentiation of human periodontal ligament stem cells through YAP-mediated mechanical memory [J]. Oral Dis, 2023, 29(1):274-284.
[29] Riquelme MA, Gu S, Hua R, et al. Mechanotransduction via the coordinated actions of integrins, PI3K signaling and connexin hemichannels [J]. Bone Res, 2021, 9(1): 8.
[30] Seetharaman S, Etienne Manneville S. Integrin diversity brings specificity in mechanotransduction [J]. Biol Cell, 2018, 110(3): 49-64.
[31] Niu HY, Lin D, Tang W, et al. Surface topography regulates osteogenic differentiation of MSCs via crosstalk between FAK/MAPK and ILK/β-catenin pathways in a hierarchically porous environment [J]. ACS Biomater Sci Eng, 2017, 3(12): 3161-3175.
[32] Coste B, Xiao B, Santos JS, et al. Piezo proteins are pore-forming subunits of mechanically activated channels [J]. Nature, 2012, 483(7388): 176-181.
[33] Hu R, Yang ZY, Li YH, et al. LIPUS promotes endothelial differentiation and angiogenesis of periodontal ligament stem cells by activating Piezo1 [J]. Int J Stem Cells, 2022, 15(4): 372-383.
[34] Gao Q, Cooper PR, Walmsley AD, et al. Role of Piezo channels in ultrasound-stimulated dental stem cells [J]. J Endod, 2017, 43(7): 1130-1136.
[35] Shen X, Wu W, Ying Y, et al. A regulatory role of Piezo1 in apoptosis of periodontal tissue and periodontal ligament fibroblasts during orthodontic tooth movement [J]. Aust Endod J, 2022.
[36] Shreberk Shaked M, Oren M. New insights into YAP/TAZ nucleo-cytoplasmic shuttling: new cancer therapeutic opportunities? [J]. Mol Oncol, 2019, 13(6): 1335-1341.
[37] LeBlanc L, Ramirez N, Kim J. Context-dependent roles of YAP/TAZ in stem cell fates and cancer [J]. Cell Mol Life Sci, 2021, 78(9): 4201-4219.
[38] Hwang JH, Lee DH, Byun MR, et al. Nanotopological plate stimulates osteogenic differentiation through TAZ activation [J]. Sci Rep, 2017, 7(1): 3632.
[39] Wang Y, Hu B, Hu R, et al. TAZ contributes to osteogenic differentiation of periodontal ligament cells under tensile stress [J]. J Periodontal Res, 2020, 55(1):152-160.