Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells

doi:10.13701/j.cnki.kqyxyj.2024.04.006

Abstract

Abstract: Objective: To investigate the effects of ipriflavone (IP) on proliferation and mineralization of human dental pulp stem cells (hDPSCs). Methods: The hDPSCs were cultured in complete culture medium containing IP (10^-9-10^-5 mol/L) and identified. The cell activity at different time points (1, 2, 3 d) was detected by CCK-8. After induced for 7 days with mineralization liquid containing IP (10^-8-10^-5 mol/L), the alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, and RT-qPCR were used to detect the osteogenic differentiation of hDPSCs. Results: CCK-8 detection showed that 10^-9-10^-5 mol/L IP could promote the proliferation of hDPSCs,and 10^-6 mol/L IP had the best results (P<0.05). In 10^-6 mol/L IP group, the ALP staining was deepened, the activity was increased (P<0.05), and the mineralization nodules were increased. RT-qPCR showed that the contents of Runt-related transcription factor, ALP, and osteocalcin in the 10^-6 mol/L IP group were significantly up-regulated (P<0.05). Conclusion: 10^-6 mol/L IP can promote the proliferation and mineralization of hDPSCs.

Key words: dental pulp stem cells, multiplication, differentiation of bone, ipriflavone

LE Manni, WANG Xiaocong, HUANG Zixuan, ZHANG Huilin, ZHANG Xiaoyue, ZHAO Qing, LI Ming, WANG Jidong. Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2024, 40(4): 310-314.

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