Porphyromonas Gingivalis Induce Vascular Smooth Muscle Cells Calcification though Regulation of TRAF6/Runx2 Pathway

doi:10.13701/j.cnki.kqyxyj.2024.05.005

Abstract

Abstract: Objective: To explore the regulatory effect of tumor necrosis factor receptor-associated factor 6 (TRAF6)/Runt-related-transcription factor-2 (Runx2) signaling axis on porphyromonas gingivalis (P. gingivalis)-induced calcification of vascular smooth muscle cells (VSMC). Methods: Heat-inactivated P. gingivalis and VSMC were co-cultured, and the calcification deposition and calcium content of VSMC were detected after 21 days. Gene and protein expression of VSMC contraction markers alpha smooth muscle actin (α-SMA) and smooth muscle 22 alpha (SM22α), as well as osteogenic markers alkaline phosphatase (ALP), osteocalcin (OC), and type I collagen A1 (Col ⅠA1) were detected. In addition, the expression of TRAF6 and Runx2 after P. gingivalis induced VSMC was detected, and its effect on Runx2 expression and calcification of VSMC and aorta was further tested by inhibiting TRAF6. Results: P. gingivalis could promote calcification deposition and increase calcium content in VSMC (P<0.05); and P. gingivalis could significantly promote the expression of osteogenic markers and inhibit the expression of VSMC contraction markers (P<0.05). Inhibition of TRAF6 could reduce the expression of Runx2, calcification deposition of VSMC and aortic wall, and the calcium content in VSMC (P<0.05). Conclusion: P. gingivalis promotes the expression of osteogenic markers in VSMC, inhibits the expression of contractile markers, and ultimately induces VSMC calcification. P. gingivalis can induce VSMC and aortic calcification through the TRAF6/Runx2 signaling axis.

Key words: P. gingivalis, vascular smooth muscle cell, vascular calcification, periodontitis, atherosclerosis

TANG Lu, YANG Wenwen, ZHANG Xianli, ZHANG Xinran, XUE Dong, LENG Lijun, ZHAO Ying. Porphyromonas Gingivalis Induce Vascular Smooth Muscle Cells Calcification though Regulation of TRAF6/Runx2 Pathway[J]. Journal of Oral Science Research, 2024, 40(5): 401-406.

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