Effect of Endogenous Hyaluronan on Proliferation of Mouse Dental Papilla Cells

doi:10.13701/j.cnki.kqyxyj.2024.07.005

Abstract

Abstract: Objective: To explore the effect of endogenous hyaluronan (HA) remodeling on the proliferation of mouse dental papilla cells (mDPCs). Methods: Fast-proliferating cells were labeled with EdU by intraperitoneal injection into mice at postnatal day 2.5. mDPCs were isolated, and then treated with different concentrations of hyaluronidase (HAase). The cell proliferation was detected by CCK-8 assay and EdU assay. Flow cytometry was used to detect cell cycle distribution and apoptosis. The transfection of siHyal2 caused HA accumulation and the cell proliferation was detected by EdU assay. Results: HA expression was mainly observed in the slow-proliferating mesenchymal cells in mouse incisors, while the expression of HA was decreased in transit-amplifying cells (TACs). The CCK-8 results indicated that 800 μg/mL HAase significantly promoted cell proliferation compared with the control group (P＜0.0001). After 48 h of HAase treatment, the EdU assay and flow cytometry results showed an increase in cellular DNA synthesis and a significant increase in the ratio of S-phase cells (P＜0.01). Additionally, early cell apoptosis was inhibited (P＜0.05). In contrast, the accumulation of HA around mDPCs was found to significantly inhibit cell proliferation (P＜0.01). Conclusion: The proliferation of mDPCs is closely related to endogenous HA. The degradation of HA promotes cell proliferation, which is inhibited by local HA accumulation.

Key words: extracellular matrix, hyaluronan, hyaluronidase 2, cell proliferation

HUANG Haiyan, YAN Yanhong, LIU Yuan, WU Jiayan, SONG Chenyu, JIANG Beizhan. Effect of Endogenous Hyaluronan on Proliferation of Mouse Dental Papilla Cells[J]. Journal of Oral Science Research, 2024, 40(7): 593-598.

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