Abstract: Objective: To explore the association of Helicobacter pylori (HP) infection with periodontal disease (PD) risk in nonsmoking Chinese Han population. Methods: Eighty-five PD patients and 95 healthy controls from June 2013 to March 2015 were included in this study. Urease Activity Assay Kit was used to detect the positive rate of HP infection in all of the participants. Differences of basic features and the association of HP infection and PD risk were calculated by SPSS19.0. Age, gender, drinking, and diabetes history were used as confounding factors to adjust the results. Results: Basic features were significantly different between case and control groups. Association analysis showed that high HP positive rate could increase the PD risk by approximately 3.23 folds (OR=3.23,95%CI=1.63-6.41). After adjusted by age, gender, drinking and diabetes history, the association was also statistically significant (OR=2.47, 95%CI=1.35-4.52). Conclusion: PD was significantly associated with the HP infection. HP might act as an independent and significant marker for PD.