Abstract: Objective: To explore the effects of MT01 on the differentiation of osteoblasts by determining the expression of specific osteoblast-related factors in MG63 infected by porphyromonas gingivalis. Methods: Human osteoblast-like cells MG63 were seeded into 6 well plates. Specific sequence oligodeoxynucleotide MT01 at a concentration of 1 μg·mL^-1 and the same volume of PBS were added separately, and the cells were incubated for 3 hours. Then bacterium suspension of P.g at multiplicity of infection MOI=100∶1 was added. The same volume of PBS was added in the control group. Four groups were set, i.e. MT01+P.g, MT01, P.g, and blank control group. Real-time quantitative polymerase chain reaction was used to detect the mRNA expression of osteoblast-related factors Runx2, SP7 at 2, 4, 6, 8, 12, and 24 hours respectively. Results: The mRNA levels of osteoblast-related factors in MG63 were markedly upregulated after adding MT01 following infected by P.g or not. However, the ability of MT01 to upregulate the expression of Runx2 and SP7 at different time-points was different. Conclusion: MT01 could promote the differentiation of osteoblasts in infected status.