Abstract: Objective: To compare the effect of modification of titanium plate with fibronectin (Fn) using three fluoroethane sulfonyl chloride and silane. Methods: Three fluoroethane sulfonyl chloride and silane were used to bond Fn on the micro grooves titanium surface. XPS was applied to analyze and compare the surface elements of different materials. Fluorescence staining was used to observe the quantity and distribution of protein on different material surfaces. DAPI staining was used to detect the early attachment of cells after HGF was seeded on different material surfaces for 2h, 4h, and 6h. The cell proliferation rates were compared by CCK-8 assay after HGF was seeded on the surface for 6h, 12h, 1d, 3d, 5d, 7d. Results: XPS results indicated that the protein content of the surface of the silane group was higher than that of the three fluorine group and the blank group (P<0.05). The content of silane group suggested that fibronectin surface immunofluorescence was higher than that of three fluorine group and blank control group (P<0.05). DAPI staining indicated that the number of cells attached to the three groups was comparable. However, after 4h and 6h, the number of cells on the surface of silane group was significantly higher than that of other two groups (P<0.05). CCK-8 results indicated that the cell proliferation rate of silane group was higher than those of three fluorine group and blank group at six time periods (P<0.05). Conclusion: The effect of silane modified method is better than three fluoroethane sulfonyl chloride to bond fibrin on the titanium plate.

Key words: Silane, Tresyl chloride, Fibronectin, Gingival fibroblast