Expression of Long Non-coding RNA AFAP1-AS1 and Its Clinicopathologic Significance in Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2016.09.022

Abstract

Abstract: Objective: To investigate the expression of long non-coding RNA (lncRNA) (actin filament-associated protein 1-antisense RNA1, AFAP1-AS1) and its association with clinicopathologic features in oral squamous cell carcinoma (OSCC). Methods: The expression of AFAP1-AS1 was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR) in 75 tumor tissues of OSCC patients and matched adjacent tissues. The correlation between AFAP1-AS1 expression and clinicopathologic features of OSCC patients was analyzed. LncRNA AFAP1-AS1 siRNA was transfected into Tca8113 cells, and CCK-8 kit was used to detect the cell proliferation. Results: The relative expression levels of lncRNA AFAP1-AS1 in OSCC tissues were 5.16 times of that in adjacent normal tissues (P＜0.01). High AFAP1-AS1 expression was associated with cell differentiation, lymphatic metastasis and pathological stage of OSCC (P＜0.05). Furthermore, inhibition of AFAP1-AS1 expression suppressed the proliferation of human tongue carcinoma Tca8113 cells (P＜0.01). Conclusion: Up-regulation of AFAP1-AS1 in tumor tissue might act as an oncogene and promote the development of OSCC.

Key words: Oral squamous cell carcinoma, LncRNA, AFAP1-AS1, Proliferation

CLC Number:

R739.8

FU Cong , ZHANG Xing-wei. Expression of Long Non-coding RNA AFAP1-AS1 and Its Clinicopathologic Significance in Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2016, 32(9): 983-986.

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