17β-Estradiol Induces Odontoblastic Differentiation of Dental Pulp Stem Cells through Activation of Runx2

doi:10.13701/j.cnki.kqyxyj.2017.10.005

Abstract

Abstract: Objective: To investigate the mechanism of odontogenic differentiation of DPSCs by regulation of 17β-estradiol (E2). Methods: hDPSCs cultured in vitro were stimulated with 1μM E2, and then DPSCs were measured by qRT-PCR, western blot, and immunofluorescence to investigate the expressions of the odontogenic differentiation marker genes and related downstream regulators, and to clarify the mechanism that E2 regulated the odontogenic differentiation of DPSCs.Results: E2 increased the expressions of DSPP and DMP1 mRNA and ALP activity, enhanced the staining intensity of DSPP, and promoted the odontogenic differentiation of DPSCs. During the E2-induced odontogenic differentiation of DPSCs, E2 activated the Runx2 activity. RNA interference of Runx2 obviously inhibited the expressions of DSPP and DMP1 mRNA and the DSPP staining intensity. Conclusion: E2 promotes the odontogenic differentiation of DPSCs partly through Runx2 activity.

Key words: 17β-estradiol Dental, pulp stem cells, Cell differentiation, Runx2

CLC Number:

R780

JI Yang, KONG Tao, TANG Xue-peng, LI Shi-ting,JI Yang. 17β-Estradiol Induces Odontoblastic Differentiation of Dental Pulp Stem Cells through Activation of Runx2[J]. Journal of Oral Science Research, 2017, 33(10): 1040-1043.

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