Effect of Lentivirus Mediated BMP-2 and VEGF-165 Transfection of Goat Bone Marrow Mesenchymal Stem Cells into Cartilage Differentiation

doi:10.13701/j.cnki.kqyxyj.2020.06.007

Abstract

Abstract: Objective: To investigate the effect of lentivirus-mediated BMP-2 and VEGF-165 transfection of goat bone marrow mesenchymal stem cells into cartilage differentiation. Methods: The primary goat bone marrow mesenchymal stem cells were cultured by whole bone marrow adherent method. The target gene was transfected and mediated by virus to study the feasibility of goat BMSCs as the gene co-transfer target cell and the biological changes of cells in vitro, such as culture conditions, proliferation, and differentiation into cartilage after transfection. Results: The expression of BMP-2 mRNA in transfected group was not significantly different from that in BMP-2 group, and the same as the expression of VEGF-165mRNA in transfected group to that of VEGF-165 group. The expression of OCN mRNA in Lv-BMP2-VEGF165 transfection group was higher than that in single gene transfection group (P<0.05), and BMP2 and VEGF165 proteins were only highly expressed in Lv-BMP2-VEGF165 group. The expression of OCN protein in Lv-BMP2-VEGF165 group was significantly higher than that in other groups (P<0.01), and the activity of ALP in Lv-BMP2-VEGF165 transfection group was significantly higher than those of other groups. Conclusion: BMP2, VEGF165, and BMP2/VEGF-165 can be successfully transfected into goat bone marrow mesenchymal stem cells and can be stably expressed for a long time. The synergistic effect of BMP2/VEGF-165 could promote the production of ALP and the synthesis of osteocalcin, and induce the cells into cartilage.

Key words: goat bone marrow mesenchymal stem cells, bone morphogenetic protein 2, vascular endothelial growth factor 165, transfection, tissue engineering, alkaline phosphatase, osteocalcin

WANG Jing, Tayier·ALIPU, YAO Zhitao, Adilijiang·SAIMAITI, ZHU Xiaoyu, SUN Changhao, Maimaitituxun·TUERDI. Effect of Lentivirus Mediated BMP-2 and VEGF-165 Transfection of Goat Bone Marrow Mesenchymal Stem Cells into Cartilage Differentiation[J]. Journal of Oral Science Research, 2020, 36(6): 528-533.

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