Effect of Emodin on Proliferation, Invasion and Migration of Oral Squamous Carcinoma Cells

doi:10.13701/j.cnki.kqyxyj.2021.11.017

Abstract

Abstract: Objective: To investigate the effect of emodin on proliferation, invasion, and migration of human oral squamous cell carcinoma cells CAL-27 and SCC-15 and its molecular mechanism. Methods: CAL-27 and SCC-15 cells were treated with different concentrations of emodin. CCK-8 was used to detect their proliferation activity. According to the results, the corresponding drug concentration (IC50) was calculated when the cell inhibition rate was 50% at different time. Scratch test and Transwell invasion test were used to detect the effects of emodin on the migration and invasion of two kinds of cells. Western blot was used to detect the effect of emodin on the expression of MMP-2 and MMP-9. Real time quantitative PCR (qRT-PCR) was used to detect the effect of emodin on the expression of MMP-2 and MMP-9 mRNA. Results: Compared with the control group, emodin at different concentrations could inhibit the proliferation of CAL-27 and SCC-15 cells (P<0.05) in a time concentration dependent manner. Compared with the control group, Rhein could significantly reduce the wound healing rate and the number of invasive cells (P<0.05), which was concentration dependent. Compared with the control group, the mRNA and protein expression levels of MMP-2 and MMP-9 in emodin treated cells were significantly decreased (P<0.05). Conclusion: Emodin can significantly inhibit the proliferation, invasion, and migration of human oral squamous cell carcinoma cells in vitro, which may relate to the down regulation of the expression of MMP-2 and MMP-9.

Key words: emodin, oral squamous carcinoma cells, proliferation, invasion, migration

LIU Yang, ZHANG Hao, YU Wei, HU Tuqiang, TANG Weiwei. Effect of Emodin on Proliferation, Invasion and Migration of Oral Squamous Carcinoma Cells[J]. Journal of Oral Science Research, 2021, 37(11): 1042-1047.

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