Effects of Fam83h Mutation on Proliferation and Migration of Mouse Osteoblasts

doi:10.13701/j.cnki.kqyxyj.2023.05.011

Abstract

Abstract: Objective: To explore the effect of Fam83h mutation on proliferation and migration of mouse osteoblasts (MOB) and the related mechanism. Methods: The whole body of wild-type and Fam83h-mutated mice were analyzed by micro-CT. CCK-8 and wound healing were detected in MOBs isolated from 3-day-old wild-type and Fam83h-mutated mice. RNA-seq was performed to compare the transcriptional profile of MOBs of Fam83h-mutated mice with wild-type mice. Phosphorylation of AKT1-S473 and AKT1 were measured by Western blot. Results: Fam83h-mutated mice showed decreased bone volume fraction (P＜0.001). Fam83h mutation significantly repressed cell proliferation and migration in MOBs (P<0.01). KEGG pathway analysis of differentially expressed genes (DEGs) identified PI3K/AKT signaling pathway. Furthermore, the protein expression level of p-AKT1-S473 was decreased in MOBs of Fam83h mutation mice. Conclusion: Fam83h mutation may inhibit the proliferation and migration of MOBs through PI3K/AKT signaling pathway, resulting in bone mass loss in Fam83h-mutated mice

Key words: Fam83h mutation, osteoblast, cell proliferation, cell migration

HE Zhenru, ZHENG Xueqing, YANG Chunhui, WANG Xin, LI Yang, SONG Yaling. Effects of Fam83h Mutation on Proliferation and Migration of Mouse Osteoblasts[J]. Journal of Oral Science Research, 2023, 39(5): 435-439.

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