Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca2+ Signaling Pathway within Estrogen Microenvironment

doi:10.13701/j.cnki.kqyxyj.2019.01.008

Abstract

Abstract: Objective: To investigate the effects of estrogen on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) regulated by non-canonical Wnt/Ca²⁺ signaling pathway. Methods: The third generation of hPDLSCs were divided into blank group, control group, estrogen group, and inhibitor group. ALP activity was measured on the 7th day after osteogenesis induction, the expression of Wnt signaling pathway genes and osteoblast-related genes were detected by qRT-PCR. Results: ALP active in estrogen group and inhibitor group were higher than that in control group (P<0.05). There was no significant difference in ALP expression between estrogen group and inhibitor group (P>0.05). qRT-PCR showed that the expression of Wnt signaling pathway genes, such as β-catenin, CaMKⅡ, and NLK, and osteogenic genes (RUNX2, OCN) in estrogen group and inhibitor group were up-regulated compared with control group (P<0.05). The expression of β -catenin in inhibitor group was lower than that in estrogen group (P<0.05), while the expression of CaMKⅡ and NLK was higher (P<0.05). But osteogenic gene RUNX2 and OCN did not exhibit such difference (P>0.05). Conclusion: Estrogen promotes the osteogenic differentiation of hPDLSCs by enhancing the activation of non-canonical Wnt/Ca²⁺ signaling pathway.

Key words: Human periodontal ligament stem cells, Estrogen, Non-canonical Wnt/Ca²⁺ signaling pathway, Osteogenic differentiation

WU Xiao-ling, ZHENG Qian, LV Jia-ling, ZHAO Xian, XU Jie, YU Jing-hong, XU Xiao-mei. Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca²⁺ Signaling Pathway within Estrogen Microenvironment[J]. Journal of Oral Science Research, 2019, 35(1): 33-37.

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Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca²⁺ Signaling Pathway within Estrogen Microenvironment

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