Effect of Combination of Stromal Vascular Fraction and PRF on Autogenous Fat Transplantation: An Animal Experimental Study

doi:10.13701/j.cnki.kqyxyj.2020.12.018

Abstract

Abstract: Objective: To investigate the effect of stromal vascular fraction combined with PRF on the absorptivity of autogenous fat transplantation and the best ratio of adipose granules to PRF. Methods: The soft tissue defect model of bilateral rabbit ears was used. The volume, survival rate, and quality of the grafts were compared at different observation points. The arrangement, morphology, and quantity of adipocytes in each group were compared by HE staining and electron microscope scanning. Results: In the simple fat tissue transplantation group, there were 2 cases with cicatrization of the epidermis in the operation area, and no obvious abnormality was found in the rest. With the increase of observation time, the survival rate, mass, and volume of the grafts were gradually reduced. When the mass ratio of fat particles to PRF was 3∶1, the survival rate, mass, and volume of the grafts were the largest. With the increase of time, the PRF was slowly released, and histological observation showed that the fat cells in each group were not abnormal. With the same degree of reconstruction, the number of intact adipocytes in group B was the most, and the shape and size of adipocytes in group B were more uniform. Conclusion: The combination of PRF and stromal vascular fraction can reduce the absorption rate of autogenous adipose tissue and improve the survival rate of the graft. When the mass ratio of adipose granules to PRF was 3∶1, the graft survival rate was the highest.

Key words: PRF, stromal vascular fraction, autogenous fat transplantation, tissue engineering

LONG Jing, HUANG Xieshan, PAN Xiaomeng, LIAO Jun. Effect of Combination of Stromal Vascular Fraction and PRF on Autogenous Fat Transplantation: An Animal Experimental Study[J]. Journal of Oral Science Research, 2020, 36(12): 1162-1167.

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