IGF1 Participation in Bone Regeneration of Type 2 Diabetes Mellitus Patients through PI3K-AKT Signaling Pathway in Vitro

doi:10.13701/j.cnki.kqyxyj.2020.12.017

Abstract

Abstract: Objective: To investigate the mechanism of insulin like growth factor 1 (IGF1) in bone regeneration of type 2 diabetes mellitus (T2DM) patients in vitro. Methods: Human IGF1 ELISA TABLE was used to detect the IGF1 levels of serum between normal and T2DM patients. The difference of senescence phenotype between normal bone mesenchymal stem cells (BMSC) and T2DM BMSC (DM-BMSC) was detected by β-galactosidase senescence staining. The expression of osteogenic proteins and PI3K-AKT signaling pathway was investigated using western blotting analysis. To further illustrate the characters of senescence, osteogenesis, and PI3K-AKT signaling pathway expression, IGF1 and AKT inhibitor were added to DM-BMSC culture medium. Results: ELISA analysis showed that serum level of IGF1 was deficient in T2DM (P<0.05). The number of senescent DM-BMSC was more than that of BMSC (P<0.001). Western blotting indicated that DM-BMSC exhibited insufficient ability of osteogenesis and PI3K-AKT signaling pathway expression compared to BMSC. After cultured with IGF1, the number of senescent DM-BMSC decreased while the expression of osteogenesis markers and PI3K-AKT signaling pathway increased. However, AKT inhibitor restrained this function of IGF1. Conclusion: Lack of IGF1 leads to senescence of DM-BMSC and deficient osteogenic ability through PI3K-AKT signaling pathway in vitro which may affect bone regeneration of type 2 diabetes mellitus patients.

Key words: diabetes mellitus, insulin like growth factor 1, mesenchymal stem cells, cell aging, osteogenesis

GE Jie, ZHOU Peipei, XU Tianshu, ZHU Zhichao, YANG Xu. IGF1 Participation in Bone Regeneration of Type 2 Diabetes Mellitus Patients through PI3K-AKT Signaling Pathway in Vitro[J]. Journal of Oral Science Research, 2020, 36(12): 1157-1161.

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