Abstract: Objective: To investigate the effects of advanced glycation end products (AGEs) on the proliferation of mouse bone marrow mesenchymal stem cells (BMSCs). Methods: BMSCs were adopted by whole bone marrow adherence method. Osteogenic differentiation capacity of BMSCs was evaluated by Alizarin red staining. Adipogenic differentiation capacity of BMSCs was evaluated by Oil red staining. After induced with different concentrations of AGEs, the proliferation of BMSCs was assayed by MTT. In addition, real-time quantitative reverse transcription polymerase chain reaction (real-time PCR) was performed to detect the gene expression levels. Results: After 21-day induction, Alizarin red staining showed the formation of mineralization nodules and oil red staining showed the formation of lipid droplets. All the different concentrations of AGEs (10μg/mL, 50μg/mL, 100μg/mL, 200μg/mL) obviously inhibited the proliferation of BMSCs. Meanwhile, the expressions of P27 and cyclinD1 in the experimental group was significantly different from that in the control group (P<0.05). Conclusion: AGEs could decrease the proliferation capacity of BMSCs and regulate the gene expression levels of P27 and cyclin D1.

Key words: Advanced glycation end products, Mouse bone marrow mesenchymal stem cell, Proliferation