Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Human Periodontal Ligament Cell by Co-culture in Vitro

Abstract

Abstract: Objective: To investigate the ability of human umbilical cord mesenchymal stem cells (hUCMSCs) differentiation into human periodontal ligament cells (hPDLs) by co-culture in vitro. Methods: The hUCMSCs and hPDLs were obtained by primary culture. hUCMSCs and hPDLs were indirectly cocultured in transwell plates. The stemness markers such as CD146, sex determining region Y-Box 2 (SOX2), stage-specific embryonic antigen (SSEA), and Stro-1 changes were detected by flow cytometry after coculture for 0, 1 and 3 weeks, respectively. The Vimentin expression was determined by immunofluorescence stain and Western blot. Results: During the co-culture process, the expression of stemness markers in hUCMSCs decreased gradually, while the Vimentin expression was on the contrary. Conclusion: The hUCMSCs had the potential to differentiate into hPDLs under co-culture condition, which suggested that it could be used for periodontal repair in the future.

Key words: Umbilical cord mesenchymal stem cells, Periodontal ligament cells, Co-culture

CLC Number:

R329.2

LI Yun-peng, ZHAO Xin, WANG Yi-bo, et al. Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Human Periodontal Ligament Cell by Co-culture in Vitro[J]. Journal of Oral Science Research, 2015, 31(8): 786-.

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