Effects of EMP1 on the Proliferation, Migration and Invasion of Oral Squamous Cancer Cells in Vitro.

doi:10.13701/j.cnki.kqyxyj.2016.12.004

Abstract

Abstract: Objective: To investigate the effects of EMP1 on the proliferation and invasion of oral squamous cancer cells(OSCC) in vitro. Methods: pEGFP-N1-EMP1expression vectors were constructed and transfected stably into Tb3.1 cell. Tb3.1-pEGFP-N1-EMP1 cell, wild type Tb3.1 cell and Tb3.1-pEGFP-N1 cell were tested using MTT to assay cell proliferation. Cell cycle and invasion were analyzed by flow cytometry and invasion assay, respectively. One-way ANOVA was adopted to compare among groups. Results: Compared with wild type Tb3.1 cell and Tb3.1-pEGFP-N1 cell, the growth of Tb3.1-pEGFP-N1-EMP1 cell was inhibited, and the proportion of cells in S and G2-M phase and the number of invasive cells were reduced in Tb3.1-pEGFP-N1-EMP1 cell (P＜0.05). Conclusion: EMP1 may negatively regulate the proliferation, migration and invasion of tumor cells in the oncogenesis and development of OSCC.

Key words: EMP1 Oral, squamous cancer cells, Transfection , Proliferation Migration , Invasion

CLC Number:

R739.8

ZHANG Jun, DAI Xiao-hua, ZOU Hui-ru, LIAN Xiao-li, LI Yan-ni.. Effects of EMP1 on the Proliferation, Migration and Invasion of Oral Squamous Cancer Cells in Vitro.[J]. Journal of Oral Science Research, 2016, 32(12): 1240-1243.

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