Expression of E-cad and CD44s in the Rat Tongue Carcinogensis.

doi:10.13701/j.cnki.kqyxyj.2017.02.010

Abstract

Abstract: Objective: To explore the expression of E-cad and CD44s in the rat tongue carcinogensis. Methods: The water drinking method of 4NQO was used in a barrier system to induce tongue carcinogensis of SD rats. Immunohistochemical technique was used to study the expression of E-cad and CD44s in 82 tissue specimens of rat tongue carcinogensis. Results: In the process of rat tongue carcinogensis, the expression of E-cad decreased while that of CD44s increased, and the differences in the expression of E-cad and CD44s were statistically significant for each group (P<0.05). The expression of E-cad had a significantly negative correlation with CD44s expression (r_s=-0.675, P<0.001). Conclusion: E-cad and CD44s were involved in the occurrence and development of tongue squamous cell carcinoma, and combined detection of CD44s and E-cad may provide a new method for early clinical diagnosis.

Key words: Tongue squamous cell carcinoma, E-cad , CD44s

CLC Number:

R739.8

LIU Qiong-yao, LIAO Peng-cheng, TAN Hong, ZHANG Xu, WANG Min, NIE Min-hai. Expression of E-cad and CD44s in the Rat Tongue Carcinogensis.[J]. Journal of Oral Science Research, 2017, 33(2): 158-161.

References

[1] Petersen PE. The World Oral Health Report 2003: continuous improvement of oral health in the 21st century-the approach of the WHO Global Oral Health Programme[J]. Community Dent Oral Epidemiol, 2003, 31(1)∶3-23
[2] Diniz-Freitas M, Garc a-Caballero T, Ant nez-L pez J, et al. Reduced E-cadherin expression is an indicator of unfavourable prognosis in oral squamous cell carcinoma[J]. Oral Oncol, 2006, 42(2)∶190-200
[3] Luo SL, Xie YG, Li Z, et al. E-cadherin expression and prognosis of oral cancer: a meta-analysis[J]. Tumour Biol, 2014, 35(6)∶5533-5537
[4] Kosunen A,Pirinen R,Ropponen K, et al.CD44 expression and its relationship with MMP-9, clinicopathological factors and survival in oral squamous cell carcinoma[J]. Oral Oncol, 2007, 43(1)∶51-59
[5] Satoa S, Miyasuchia M, Takekoshia T, et al. Reduced expression of CD44 variant 9 is related to lymph node metastasis and poor survival in squamous cell carcinoma of tongue [J]. Oral Oncol, 2000, 36(4)∶545-549
[6] Pedrazzani C, Corso G, Marrelli D, et al. E-cadherin and hereditary diffuse gastric cancer[J]. Surgery, 2007, 142(5)∶645-657
[7] Stella M R, Virginia L, Susana G, et al. Expression of CD44s and CD44 splice variants in humanmelanoma [J]. Oncol Rep,2002,9(1)∶51-56
[8] 缪克红.口腔扁平苔藓和腔鳞状细胞癌组织中HERG1、KV3-4钾离子通道蛋白表达的研究.[学位论文],2011;泸州医学院:口腔临床医学
[9] Holcomb K, Delatorre R, Pedemonte B,et al. Bader Pedemonte, E-Cadherin Expression in Endometrioid, Papillary Serous, and Clear Cell Carcinoma of the Endometrium[J]. Obstet Gynecol, 2002, 100(6)∶1290-1295
[10] Sridevi U, Jain A, Nagalaxmi V, et al. Expression of E-cadherin in normal oral mucosa, in oral precancerous lesions and in oral carcinomas [J]. Eur J Dent,2015,9(3)∶364-372
[11] Afify A, Purnell P, Nguyen L. Role of CD44s and CD44v6 on human breast cancer cell adhesion, migration, and invasion[J]. Exp Mol Pathol, 2009, 86(2)∶95-100
[12] Liu YJ, Yan PS, Li J, et al. Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer[J]. World J Gastroenterol, 2005, 11(42)∶6601-6606
[13] Ashida K, Terada T, Kitamura Y, et al. Expression of E-cadherin, alpha-catenin, beta-catenin, and CD44 (standard and variant isoforms) in human cholangiocarcinoma: an immunohistochemical study [J]. Hepatology, 1998, 27(4)∶974-982

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