Comparison of Viability and Apoptosis of Gingival Fibroblasts in the Patients with Chronic Periodontitis and Healthy People.

doi:10.13701/j.cnki.kqyxyj.2016.05.012

Abstract

Abstract: Objective: To investigate the differences in the proliferation and apoptosis of human gingival fibroblasts (HGFs) between patients with chronic periodontitis and healthy people and provide experimental evidence for the susceptibility of chronic periodontitis. Methods: HGFs were obtained from patients with chronic periodontitis and healthy people and cultured in vitro. Cell activity between two groups was detected by MTT assay. Real-time PCR was used to detect the expression levels of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax in HGFs from the two groups, and the ratio of Bcl-2 to Bax was compared. Results: Compared with the healthy group, the cell activity of the HGFs in chronic periodontitis group decreased significantly after 4 days culture (P<0.05). The ratio of Bcl-2/Bax significantly decreased after 2 days and 4 days in vitro in a time-dependent manner (P<0.05). Conclusion: HGFs cells from patients with chronic periodontitis show low cell activity and high cell apoptosis, which indicates the susceptibility of periodontitis.

CLC Number:

R781.4

DONG Yun-yun, SONG Li-ting, ZHU Dong-wang, DENG Jia-yin, JIANG Shao-yun.. Comparison of Viability and Apoptosis of Gingival Fibroblasts in the Patients with Chronic Periodontitis and Healthy People.[J]. Journal of Oral Science Research, 2016, 32(5): 474-477.

References

[1] Loe H, Anerud A, Boysen H, et al.Natural history of periodontal disease in man. Rapid, moderate and no loss of attachment in Sri Lankan laborers 14 to 46 years of age [J]. J ClinPeriodontol. 1986, 13(5)∶431-445
[2] Herath TD, Wang Y, Seneviratne CJ, et al.The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts [J]. BMC Microbiol, 2013, 13∶73-77
[3] Garcia-Jove Navarro M, Basset C, Arcondeguy T, et al. Api5 contributes to E2F1 control of the G1/S cell cycle phase transition [J]. PLoS One, 2013, 8(8)∶e71443
[4] 孟焕新.牙周病学[M].第4版.北京:人民卫生出版社, 2012∶169-170
[5] Armitage GC.Diagnosis of periodontal diseases [J]. J Periodontol. 2003, 74(8)∶1237-1247
[6] 潘春玲, 赵海礁, 谭丽思, 等. 牙龈卟啉单胞菌感染MG63细胞对细胞周期的影响[J].口腔医学研究, 2015,31(9)∶863-866
[7] Gamonal J, Bascones A, Acevedo A, et al. Apoptosis in chronic adult periodontitis analyzed by in situ DNA breaks, electron microscopy, and immunohistochemistry [J]. J Periodontol, 2001, 72(4)∶517-525
[8] Lalier L, Cartron PF, Juin P, et al. Bax activation and mitochondrial insertion during apoptosis [J]. Apoptosis, 2007, 12(5)∶887-896
[9] Tor YS, Yazan LS, Foo JB, et al. Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile [J]. PLoS One, 2015, 10(6)∶e0127441
[10] Korsmeyer SJ. BCL-2 gene family and the regulation of programmed cell death [J]. Cancer Res. 1999, 59(7)∶1693s-1700s
[11] Korsmeyer SJ, Shutter JR, Veis DJ, et al. Bcl-2/Bax: a rheostat that regulates an anti-oxidant pathway and cell death [J]. Semin Cancer Biol, 1993, 4(6)∶327-332
[12] Ren WW, Chen SL, Qiu J, et al. Role and mechanism of bcl-2 and bax in highglucose-mediated apoptosis of human periodontalligament cells [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51)∶8254-8260
[13] Shroff EH, Snyder C, Chandel NS. Role of Bcl-2 family members in anoxia induced cell death [J]. Cell Cycle, 2007, 6(7)∶807-809
[14] Chrysis D, Calikoglu AS, Ye P,et al. Insulin-like growth factor-I overexpression attenuates cerebellar apoptosis by altering the expression of Bcl family proteins in a developmentally specific manner [J]. J Neurosci, 2001, 21(5)∶1481-1489

[1]	WU Yun, ZHAO Yan, JI Yao-ting, XIA Hai-bing, ZHANG Bi, WANG Yi-ning. Role of C4orf7 in Osteogenic Differentiation of Human Periodontal Ligament Cells [J]. Journal of Oral Science Research, 2018, 34(2): 125-129.
[2]	TANG Lu, XUE Dong, BAI Yu-hao, WANG Peng-cheng, LI Wen-ting, DONG Yan, ZHOU Wei, ZENG Hong-yan, ZHAO Ying. Calcification of Vascular Smooth Muscle CellInduced by Porphyromonasgingivalis. [J]. Journal of Oral Science Research, 2018, 34(2): 130-133.
[3]	LIU Xue-jiao, ZHANG Zhen, LI Cheng-zhang, SHANG Shu-huan. Effect of Wnt/β-catenin Signaling Pathway on Expressions of EMMPRIN/MMPs in Two Cell Culture Models [J]. Journal of Oral Science Research, 2018, 34(2): 134-138.
[4]	JIANG Si-qi, YIN Feng-ying, ZHANG Fan, WANG Min, XIA Hai-bin. Effect of Primary Cilia on Expression of Osteogenic-related Genes of Human Periodontal Ligament Cells [J]. Journal of Oral Science Research, 2018, 34(1): 10-13.
[5]	WEN Jing-yu, HE Hong-bing, REN Xiao-bin, JIANG Jie. Progress of Application and Research on Antioxidants and Periodontitis [J]. Journal of Oral Science Research, 2018, 34(1): 97-99.
[6]	LIU Yue-fang, ZHU Guang-xun. Research Progress of Music Therapy in Treatment of Periodontal Diseases [J]. Journal of Oral Science Research, 2018, 34(1): 100-102.
[7]	LI Ge-ge, TANG Qiu-ling, PAN Jia-hui, WANG Liu-ran, MENG Yang, YUE Yi-yun, DING Xiao-han, LIU Dong-ning, YU Wei-xian. Study on Anti-inflammatory Effects of PMX205 in Vitro [J]. Journal of Oral Science Research, 2017, 33(12): 1246-1249.
[8]	GUO Ling, ZHANG Yuan-shui, ZHAN Dan-ting, LI Wang-yang, DING Nong-le.. Effects of RAGE Inhibitor FPS-ZM1 on hPDLFs Proliferation and Migration in High Glucose. [J]. Journal of Oral Science Research, 2017, 33(12): 1258-1261.
[9]	XU Jia, YU Zhou-qing, PENG Zhi-kai, ZHEN Jie.. Effects of Nonsurgical Periodontal Therapy on Endothelial Function and Arterial Stiffness in Patients with Chronic Periodontitis. [J]. Journal of Oral Science Research, 2017, 33(12): 1332-1335.
[10]	ZHAN Dan-ting, DING Nong-le, ZHANG Yun-shui, ZHANG Hui, GUO Ling. FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE [J]. Journal of Oral Science Research, 2017, 33(11): 1156-1160.
[11]	YANG Dong, LI Hong-mei, LIU Lin-hua, DONG Wei-li. Detection of Th17 and Related Factors in Gingival Crevicular Fluid of Patients with Different Periodontal Inflammatory Status [J]. Journal of Oral Science Research, 2017, 33(11): 1161-1164.
[12]	CHENG Jing-yi, ZHANG Yun, CHENG Cai-yun, CHENG Lan, ZHANG Xiu-yin. Changes of Microarchitecture of Alveolar Bone in Experimental Periodontitis in Rats [J]. Journal of Oral Science Research, 2017, 33(11): 1165-1168.
[13]	DONG Wen-jie, WANG Yu-kun, YUE Wen-jiang. Effects of Zoledronic Acid on Biological Characteristics of Gingival Fibroblasts [J]. Journal of Oral Science Research, 2017, 33(11): 1169-1172.
[14]	DU Fei, GE Liu-ying, HE Rui, LI Chong-xi, Zeng Shui-qin, DUAN Kai-wen. Comparative Study of Periodontal Health in HIV Patients [J]. Journal of Oral Science Research, 2017, 33(11): 1173-1176.
[15]	CHEN Fan, Zhang Yong. Short-term Curative Effect of Minocycline Gel and Hyaluronic Acid Gel as Adjunctive Treatment on Chronic Periodontitis [J]. Journal of Oral Science Research, 2017, 33(11): 1177-1180.