Effect of Myricetin on Virulence Factor and Biofilm of Streptococcus Mutans

doi:10.13701/j.cnki.kqyxyj.2022.04.011

Abstract

Abstract: Objective: To study the inhibitory effect of myricetin on virulence factors and biofilm of Streptococcus mutans (S.mutans). Methods: S. mutans ATCC 25175 was used in this study. Dilution method was used to test MIC and acid tolerance. The inhibition rate on biofilm was measured by crystalline violet staining. The acid production was measured by lactic acid kit. Anthrone method was conducted to determine the production of EPS. FESEM and CLSM were used to observed the formation of biofilm after treated by myricetin. Results: The MIC of myricetin on S.mutans was 0.8 mg/mL, and the inhibitory effect of myricetin from 0.2 mg/mL to 0.4 mg/mL on the production of biofilm was obvious, while the production of lactic acid and insoluble polysaccharide significantly reduced, and the effect of myricetin at 0.4 mg/mL on bacterial acid tolerance was significantly different. Conclusion: Myricetin plays a significant part on virulence factor and biofilm of S.mutans.

Key words: myricetin, Streptococcus mutans, biofilm, virulence factor

XU Xiaoyu, GUO Mengqin, YU Haijing , LIU Yangyang, GUO Peng. Effect of Myricetin on Virulence Factor and Biofilm of Streptococcus Mutans[J]. Journal of Oral Science Research, 2022, 38(4): 344-349.

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