Effect of Chitosan/curcumin Nanocomposite on Cariogenic Ability of Streptococcus Mutans

doi:10.13701/j.cnki.kqyxyj.2022.11.012

Abstract

Abstract: Objective: To investigate the effect of chitosan/curcumin nanocomposite on cariogenic ability of Streptococcus mutans (S. mutans). Methods: Curcumin was compounded with chitosan to form chitosan/curcumin nanocomposites (CS/cur) containing different concentrations of curcumin (0, 0.01, 0.05, and 0.10 mmol/L). CS/cur was applied to S. mutans in planktonic state and biofilm state. The growth curve of S. mutans, pH change of culture, and bacterial glycolysis ability were evaluated. Crystal violet staining was utilized to evaluate the amount of S. mutans biofilm biomass, and anthrone method was used to detect the content of insoluble extracellular polysaccharide in biofilm. Finally, colony count was used to evaluate the number of bacteria in biofilm. Results: CS/cur inhibited the growth of S. mutans, and the inhibition effect of CS/cur was cur concentration related. The pH of S. mutans culture could not be reduced to the level of blank control group. Moreover, the glycolytic ability of S. mutans also decreased after CS/cur. After CS/cur was applied to S. mutans, crystal violet staining showed that the amount of biofilm formed by S. mutans decreased (P<0.05). The results of anthrone detection of extracellular polysaccharides showed that the content of insoluble extracellular polysaccharides in biofilm decreased significantly (P<0.05). Colony count showed that the number of bacteria also decreased significantly (P<0.05). Conclusion: CS/cur nanocomposite has a significant inhibitory effect on the expression of cariogenic factors of S. mutans, suggesting that CS/cur nanocomposite is expected to become a new anti-caries drug and provides a new strategy for caries prevention and treatment.

Key words: chitosan, curcumin, streptococcus mutans, cariogenic factor

MEI Jie, SHI Wanwei, GUO Ting, WU Minjing. Effect of Chitosan/curcumin Nanocomposite on Cariogenic Ability of Streptococcus Mutans[J]. Journal of Oral Science Research, 2022, 38(11): 1058-1063.

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