Effects of Platelet-rich Fibrin on Biological Function of Gingival Fibroblasts

doi:10.13701/j.cnki.kqyxyj.2024.07.008

Abstract

Abstract: Objective: To explore the effects of freeze-dried platelet-rich fibrin (FD-PRF) on human gingival fibroblasts (hGFs) and antibacterial property of FD-PRF, and to provide a new way for controlling refractory oral mucositis (OM). Methods: Cell counting kit-8 (CCK-8), flow cytometry, scratch wound, and Transwell assays were used to investigate the effects of FD-PRF on hGFs’ cycle, proliferation, and migration. Pour plate and shaking culture methods were explored to investigate the antibacterial ability of FD-PRF. Results: CCK-8 assay showed that hGFs number increased significantly with the increase of FD-PRF concentration. In cell cycle results, proportions of cells at S-G2/M phase: 5%FD-PRF group>1%FD-PRF group > control group. And proportions of cells at G1-G0 phase were the opposite. Cells migration assays suggested that FD-PRF improved migration rate of hGFs. For antibacterial function tests of FD-PRF, it was found that clones of E. coli and S. aureus were both reduced in the FD-PRF group. Conclusion: By facilitating hGFs entering the division stage, FD-PRF promotes proliferation of hGFs. FD-PRF possesses significant chemotaxis toward hGFs. Besides, FD-PRF possesses intrinsic antibacterial property. FD-PRF may promote the healing of oral mucositis.

Key words: freeze-dried platelet-rich fibrin, fibroblasts, antibacterial property, oral mucositis

WANG Yuhua, CHAI Jihua, ZHANG Haojian. Effects of Platelet-rich Fibrin on Biological Function of Gingival Fibroblasts[J]. Journal of Oral Science Research, 2024, 40(7): 611-616.

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