Role of Dental Pulp Stem Cells Conditioned Medium in Pulp Regeneration

doi:10.13701/j.cnki.kqyxyj.2025.08.005

Abstract

Abstract: Objective: To explore the role of dental pulp stem cells conditioned medium (DPSCs-CM) in dental pulp regeneration and related mechanisms. Methods: DPSCs were stimulated with DPSCs-CM for 1, 3, and 5 days, and its effect on DPSCs proliferation was detected using cell counting kit-8 (CCK-8). Alkaline phosphatase (ALP) content and mineralization deposition level were observed by ALP staining and alizarin red staining. The expression level of cellular osteogenic marker genes was detected using qRT-PCR. The formation of vascularization was observed using the in vitro angiogenic assay. In the nude mouse model, human tooth root fragments were implanted subcutaneously in nude mice, and pulp regeneration was observed by histologic evaluation. Meanwhile, the protein regulation mechanism of DPSCs-CM on cell differentiation during dental pulp regeneration was deeply explored by analyzing its protein profile. Results: DPSCs-CM not only promoted the proliferation of DPSCs, but also their odontogenic differentiation properties. Compared with the control group, the DPSCs+5 d CM group induced after 4 weeks produced a more mature pulp-like structure. DPSCs-CM contained more odontogenic and angiogenic factors. Conclusion: DPSCs-CM positively promoted DPSCs proliferation, dentinogenic differentiation, and angiogenesis. These findings suggest a potential benefit of DPSCs-CM in the regeneration of dental pulp tissue.

Key words: conditioned medium, dental pulp stem cells, odontogenic differentiation, pulp regeneration

GAO Jing, WANG Yuhan, YUAN Shasha, CHEN Yao, LI Lu, SHEN Jing. Role of Dental Pulp Stem Cells Conditioned Medium in Pulp Regeneration[J]. Journal of Oral Science Research, 2025, 41(8): 664-671.

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