Biological Characterization of Ionized Collagen Scaffolds with EDC Cross-linking.

Abstract

Abstract: Objective: To investigate the effects of EDC cross-linking on the physicochemical properties of ionized collagen scaffolds. Methods: Ionized collagen scaffolds: native collagen(NC), methylation collagen(MC) and succinylated collagen(SC), were fabricated with modification of side chain and 1-ethyl-3-(3- dimethyl-aminopropyl) carbodiimide(EDC)cross-linking. Scaffolds were observed and analyzed using scanning electron microscopy(SEM), differential scanning calorimeter(DSC), Fourier transform infrared radiometer(FT-IR) and experiment of enzyme degradation in vitro. And the effects of EDC cross-linking on the physicochemical properties of collagen scaffolds were investigated. Results: The results indicated that modification of side chain had changed the surface charge of collagen, and EDC cross-linking had improved pore size, porosity, denatured temperature(Td) and ability of antienzyme degradation of NC and MC, changed chemical structure of NC and MC; but that had no influences on pore size, porosity, chemical structure of SC, and decreased Td and ability of antienzyme degradation of it. Coclusion: These data indicate that EDC cross-linking may lead to enhanced physicochemical properties of ionized collagen scaffolds. It provided experimental basis of ionized collagen using in periodontal tissue engineering scaffolds.

Key words: Tissue engineering , Carbodiimide , Collagen, Periodontal ligament fibroblasts, Periodontal tissue regeneration

CLC Number:

R781.4

WANG Li-xia, ZHAO Huan, JIN Shu-feng, et al. Biological Characterization of Ionized Collagen Scaffolds with EDC Cross-linking.[J]. Journal of Oral Science Research, 2015, 31(4): 343-348.

References

[1] 杨志明.组织工程基础与临床[M].成都:四川科学技术出版社,2000,14-25
[2] Kozlovsky A, Aboodi G, Moses O, et al. Bio-degradation of a resorbable collagen membrane(Bio-Gide)applied in a double-layer technique in rats [J]. Clin Qral Implants Res,2009,20(10)∶1116-1123
[3] Niu wen-yu, Wang heng, LinXiu-dan, et al. Study on biocompatibility of a new bovine tendon collagen membrane in vitro [J]. Chinese Journal of Stomatological Research,2012,6(2)∶154-160
[4] Lee CK,Koo KT,Kim TI,et al. Biological effects of a porcine- derived collagen membrane on intrabony defects [J]. J Periodontal Implant Sci,2010,40(5)∶232-238
[5] Bottino MC,Thomas VSchidt G, et al. Recent advances in the development of GTR/GBR membranes for periodontal regeneration-A materials perspective [J]. Dental Materials, 2012, 28(7)∶703-721
[6] Lee CH,Singla A,Lee y. Biomedical applications of collagen [J]. Int J Pharm,2001,22∶1-22
[7] Rault I,Freiv V,Herbage D, et al. Evaluation of different chemical methods for cross-linking collagen gel, films and sponges [J]. J Mater in Med,1996,7(2)∶215-221
[8] Park SN, Lee HJ, Lee KH, et al. Biological characterization of EDC-crosslinked collagen-hyaluronic acid matrix in dermal tissue restoration [J]. Biomaterials,2003,24∶1631-1641
[9] Pieper JS, Oosterhof A, Dijkstra PJ, et al. Preparaion and characterization of porous cross-linking collagenous matrices containing bioavailable chondroitin sulphate [J]. Biomaterials, 1999,20(9)∶847-858
[10] Sivakumar P,Suguna L,Chandrakasan G. Comparative Biochemistry and Physiology Part B, 2000, 125∶555-562
[11] 路易斯,C金奎拉等著,浓宝熔等译.基础组织学[M].山东科学技术出版社,1982∶85-109
[12] 蔡玉文主编.组织学与胚胎学[M].中国中医药出版社,2003∶1
[13] Gerard C,Catuogno C,Amargier-Huin C,et al. The effect of alginate,hyaluronate and hyaluronate derivatives biomaterials on synthesis of non-articular chondrocyte extracellular matrix [J]. J Mater Sci Mater Med, 2005, 16(6)∶541-551
[14] Lisignoli G, Cristino S, Piacentini A, et al. Celular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold [J]. J Biomaterials, 2005, 26(28)∶5677-5686
[15] Teparat T, Solt CW, Clamanl J, et al. Clinical comparison of bioabsothable Barriers with non-resorbable barriers in guided tissue regeneration in the treatment of human intrabony defects [J]. J Periodontol, 1998, 69(6)∶632-641
[16] Thomal DS, Villar CC, Cochran DL, et al. Tissue integration of collagen-based matrices: an experimental study in mice [J]. Clinical Oral Iplants Research,2012,12(23)∶1333-1339
[17] Olde Damink L HH , Dijkst ra PJ , Van Luyn MJA , et al. In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide [J]. Biomaterials, 1996, 17(7)∶679-684
[18] Hafemann B, Ghofrani K, Gattner H, et al. Cross-linking by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) of a collagen/ elastin membrane meant to be used as a dermal substitute: effects on physical, biochemical and biological features in vitro [J]. Materials Science-Materials in Medicine, 2001, 12(5)∶437-446
[19] 熊卓,孙磊.骨组织工程聚左旋乳酸多孔框架快速成形研究[J].清华大学学报,自然科学版,2002,42(2)∶157-160
[20] Wake MC, Patrik CW, Mikos AG. Pore morphology affects on the fibro vascular tissue growth in porous polymer substrates [J]. Cell Transplant, 1994, 3(4)∶339-343
[21] M.J.B.Wissink, R. Beernink, J.S. Pieper, et al. Binding and release of basic fibroblast growth factor from heparinized collagen matrices [J]. Biomaterials,2001,22∶2291-2299
[22] Tanaka Y, Kubotal A, Yokokural S, et al. Optical mechanical refinement of human amniotic membrane by dehydration and cross-linking [J]. Journal of Tissue Engineering and Regenerative Medicine,2012,9(6)∶731-737
[23] Cholasa RH, Hsu HP, Spectorb M. The reparative response to cross-linked collagen-based scaffolds in a rat spinal cord gap model [J]. Biomaterials, 2012, 33(7)∶2050-2059