Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2017.04.001

Abstract

Abstract: Objective:To study the transfection efficiency of water soluble grapheme on synovuial stem cells. Methods: The third generation of synovial-derived mesenchymal stem cells (SMSCs) was divided into two groups. In experimental group, cells were cultured with the concentration of 25moL/mL water soluble grapheme for 24 hours at the temperature of 37℃. For the control group, cells were cultured with common medium for 24 hours at the temperature of 37℃. The laser scanning microscope with 405nm excitation wavelength was employed to detect the cells marked by the water soluble grapheme. Results: Fluorescence spots could be observed in the cytoplasmic area of these stem cells in experimental group 24 hours later under the laser scanning microscope, and the light intensity of nucleus was relatively weak. Conclusion: Grapheme quantum dots (GQDs) are easily penetrated into the cytoplasm but not the nucleus. It is suggested that genetic disruptions might not happen after the GQDs entered the living stem cells.

Key words: SMSCs , GQDs , Tissue engineering, Cell cloning

CLC Number:

R329.2

WANG Yue, SHAO Bo, GONG Zhong-cheng, YANG Meng, HU Xin, LIU Hui, HU Lu-lu, WANG Bing.. Transfection Efficiency of Water Soluble Grapheme on Synovial Stem Cells.[J]. Journal of Oral Science Research, 2017, 33(4): 349-352.

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