Abstract: Objective: To observe the effects of HU-308 on osteoblast treated with high concentration of glucocorticoid. Methods: MC3T3-E1 cells were cultured in the high concentration of glucocorticoid (10^-6 mmol/L) and then added the HU-308. Cells in concentration of 10^-10, 10^-9, 10^-8, 10^-7, and 10^-6 mol/L of HU-308 were set as the experimental groups, and that with only glucocorticoid was set as the control group. The CCK-8 was used to detect the cell proliferation, ALP activity, and mRNA expression of Runx2 and OPG. The morphology of the cytoskeleton was observed by confocal laser scanning microscope when the MC3T3-E1 cells were cultured for 24h. Results: The results of CCK-8 showed that there was statistically significant between the control group and experimental group (P<0.05). The cell proliferation was effectively promoted in the experimental group (10^-9, 10^-8, 10^-7 and 10^-6 mol/L) in 4 d (P<0.05). ALP activity detection showed no significant difference between experimental groups and control group (P>0.05). With the concentration of HU-308 increased, cells spreading area increased and the internal frame structure was clearer. The mRNA expressions of Runx2 and OPG were significantly raised at low concentration of HU-308 (P<0.05), however, inhibited at high concentration (P<0.05). Conclusion: HU-308 promoted the proliferation, adhesion, and extension of MC3T3-E1 cells under the action of high concentration of glucocorticoids, and low concentration of HU-308 could promote the expression of Runx2 and OPG mRNA.

Key words: HU-308, Glucocorticoids, MC3T3-E1, Proliferation