Effect of Oral Lichen Planus-associated Myofibroblasts on Migration and Tube-formation of Vascular Endothelial Cells

doi:10.13701/j.cnki.kqyxyj.2019.08.020

Abstract

Abstract: Objective: To observe the effect of oral lichen planus (OLP)-associated myofibroblasts (MFs) on the migration and tube-formation of vascular endothelial cells, and to elucidate the role of OLP-MFs in the angiogenesis of oral lichen lesions. Methods: Tissues were obtained from the OLP patients. The specimens were analyzed using the primary separation culture methods. Cultures at passage three were used for the cellular identification of OLP-MFs. Normal fibroblasts and untreated HUVEC were set as the control group. Transwell cell migration assay was used to detect the migration of OLP-MFs after co-cultured with HUVEC. Endothelial tube formation assay was used to detect the tube formation of OLP-MFs. Results: Compared with the control groups, OLP-MFs cells highly expressed α-SMA and had the related characteristics of myofibroblasts. Considered the migration cell number was 100% in HUVEC group, NFs + HUVEC group was (114.86±8.02)%, OLP-MFs + HUVEC group was (169.83±7.58)%, and OLP-MFs significantly promoted the migration of HUVEC. The tube length of OLP-MFs + HUVEC was (159.90±12.98)%, and the control groups were (100.38±10.54)% and 100%, respectively. Conclusion: The activated fibroblasts exist in the microenvironment of oral lichen planus, and can significantly promote HUVEC migration and tube length formation.

Key words: oral lichen planus, associated myofibroblasts, vascular endothelial cells, migration, tube formation

MENG Wenxia, FENG Lu, LIU Shuguang, ZHOU Huixi, LI Zhiqiang. Effect of Oral Lichen Planus-associated Myofibroblasts on Migration and Tube-formation of Vascular Endothelial Cells[J]. Journal of Oral Science Research, 2019, 35(8): 806-809.

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