Experimental Study on Regulation of Dnmt3b on Multidirectional Differentiation of Condylar Fibrocartilage Stem Cells

doi:10.13701/j.cnki.kqyxyj.2024.06.006

Abstract

Abstract: Objective: To investigate the effect of DNA methyltransferase 3b enzyme (Dnmt3b) on adipogenic, osteogenic, and chondrogenic differentiation of rat condylar fibrocartilage stem cells (FCSCs). Methods: Rat condylar FCSCs were isolated and cultured in vitro. After using lentivirus to overexpress Dnmt3b in FCSCs, the cells were induced by adipogenesis, osteogenesis, and chondrogenesis. The expression of related genes to adipogenesis, osteogenesis, and chondrogenesis were detected. Results: Rat condylar FCSCs had the characteristics of mesenchymal stem cells. Compared with the control group, the expression of adipogenic gene peroxisome proliferator-activated receptorγ (Ppar-γ) in Dnmt3b overexpression group was decreased significantly, the expression of osteogenic gene Runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) was increased significantly, and the expression of chondrogenic gene SRY-related high mobility group-box gene 9 (Sox9) and aggrecan (ACAN) was increased significantly. Conclusion: Dnmt3b can promote chondrogenic and osteogenic differentiation of rat condylar FCSCs and inhibit their adipogenic differentiation.

Key words: DNA methyltransferase 3b enzyme, condyle fibrocartilage stem cells, adipogenic differentiation, osteogenic differentiation, chondrogenic differentiation

YANG Hui, SU Jiansheng, ZHOU Yue. Experimental Study on Regulation of Dnmt3b on Multidirectional Differentiation of Condylar Fibrocartilage Stem Cells[J]. Journal of Oral Science Research, 2024, 40(6): 501-505.

References

[1] Derwich M, Górski B, Amm E, et al. Oral glucosamine in the treatment of temporomandibular joint osteoarthritis: A systematic review [J]. Int J Mol Sci, 2023, 24(5): 4925.
[2] 秦浩洋,黄顺,季平,等.Yap表达抑制对髁突软骨干细胞增殖和凋亡的影响[J].口腔医学研究,2021,37(11): 1017-1022.
[3] Bi R, Chen K, Wang Y, et al. Regulating fibrocartilage stem cells via TNF-α/Nf-κB in TMJ osteoarthritis [J]. J Dent Res, 2022, 101(3): 312-322.
[4] Mattei AL, Bailly N, Meissner A. DNA methylation: a historical perspective [J]. Trends Genet, 2022, 38(7): 676-707.
[5] 陈梦园,游雪娇,袁必锋,等. DNA甲基化修饰的定位分析方法研究进展[J].分析测试学报,2022,41(1): 50-57.
[6] Lauria A, Meng G, Proserpio V, et al. DNMT3B supports meso-endoderm differentiation from mouse embryonic stem cells [J]. Nat Commun, 2023, 14(1): 367.
[7] Miranda-Duarte A, Borgonio-Cuadra VM, González-Huerta NC, et al. DNA methyltransferase genes polymorphisms are associated with primary knee osteoarthritis: a matched case-control study [J]. Rheumatol Int, 2020, 40(4): 573-581.
[8] Shen J, Wang C, Li D, et al. DNA methyltransferase 3b regulates articular cartilage homeostasis by altering metabolism [J]. JCI Insight, 2017, 2(12): e93612.
[9] Xiong S, Zhao Y, Xu T. DNA methyltransferase 3 beta mediates the methylation of the microRNA-34a promoter and enhances chondrocyte viability in osteoarthritis [J]. Bioengineered, 2021, 12(2): 11138-11155.
[10] Fan Y, Cui C, Li P, et al. Fibrocartilage stem cells in the temporomandibular joint: Insights from animal and human studies [J]. Front Cell Dev Biol, 2021, 9: 665995.
[11] Matheus HR, Özdemir ŞD, Guastaldi FPS. Stem cell-based therapies for temporomandibular joint osteoarthritis and regeneration of cartilage/osteochondral defects: a systematic review of preclinical experiments [J]. Osteoarthritis Cartilage, 2022, 30(9): 1174-1185.
[12] Embree M, Chen M, Pylawka S. Exploiting endogenous fibrocartilage stem cells to regenerate cartilage and repair joint injury [J]. Nat Commun, 2016, 7: 13073.
[13] Bi R, Yin Q, Mei J, et al. Identification of human temporomandibular joint fibrocartilage stem cells with distinct chondrogenic capacity [J]. Osteoarthritis Cartilage, 2020, 28(6): 842-852.
[14] Dura M, Teissandier A, Armand M, et al. DNMT3A-dependent DNA methylation is required for spermatogonial stem cells to commit to spermatogenesis [J]. Nat Genet, 2022, 54(4): 469-480.
[15] Shen WC, Lai YC, Li LH, et al. Methylation and PTEN activation in dental pulp mesenchymal stem cells promotes osteogenesis and reduces oncogenesis [J]. Nat Commun, 2019, 10(1): 2226.
[16] Cieslar-Pobuda A, Ahrens TD, Caglayan S, et al. DNMT3B deficiency alters mitochondrial biogenesis and α-ketoglutarate levels in human embryonic stem cells [J]. Stem Cells, 2020, 38(11): 1409-1422.
[17] Zhou Y, Chen M, O’Keefe RJ, et al. Epigenetic and therapeutic implications of dnmt3b in temporomandibular joint osteoarthritis [J]. Am J Transl Res, 2019, 11(3): 1736-1747.