Study on Mechanism of Huoxue Huayu Prescription in the Treatment of Oral Lichen Planus Based on GEO Data Mining and Network Pharmacology

doi:10.13701/j.cnki.kqyxyj.2022.05.015

Abstract

Abstract: Objective: To study the key components, potential targets, and mechanisms of Huoxue Huayu prescription in the treatment of oral lichen planus based on GEO data mining and network pharmacology. Methods: The GSE52130 data set in GEO database was crawled by R language, and differential therapeutic targets of OLP were analyzed. The chemical constituents and targets of Huoxue Huayu prescription were obtained based on TCMSP database, and the composition-target network and protein-protein interaction network were plotted using Perl software, Cytoscape software, and String. The GO and KEGG analysis of key target genes were completed by R language. Results: There were 28 key chemical components of Huoxue Huyu prescription in OLP treatment, including quercetin, kaempferol, stigmasterol, etc. And 13 core targets were found, including IL-1β, PTGS2 (COX-2), MAO, et al. KEGG analysis showed that Huoxue Huayu prescription could affect neurotransmitter metabolism, cytomegalovirus infection, and AGE-RAGE pathways. Conclusion: The result provided theoretical reference for the subsequent in depth study of TCM treatment of OLP.

Key words: Huoxue Huayu prescription, oral lichen planus, network pharmacology, target, treatment mechanism

YANG Qian, LAI Renfa, LIU Xiangning. Study on Mechanism of Huoxue Huayu Prescription in the Treatment of Oral Lichen Planus Based on GEO Data Mining and Network Pharmacology[J]. Journal of Oral Science Research, 2022, 38(5): 465-470.

References

[1] Mollaoglu N. Oral lichen planus: a review [J]. Br J Oral Maxillofac Surg, 2000, 38(4):370-377.
[2] Villarroel Dorrego M, Correnti M, Delgado R, et al. Oral lichen planus: immunohistology of mucosal lesions [J]. J Oral Pathol Med, 2002, 31(7): 410-414.
[3] Liao H, Luo Y, Long L, et al. Anxiety and oral lichen planus [J]. Oral Dis, 2021, 27(3):506-514.
[4] Villanueva-Sánchez FG, Escalante-Macías LH, Zambrano-Galván G, et al. Oral lichen planus: Case report and literature review [J]. Rev Alerg Mex, 2018, 65(4):424-430.
[5] 刘国华.活血化瘀方联合单唾液酸四己糖神经节苷脂钠注射液治疗急性脑出血的效果[J].河南医学研究,2019,28(19):3575-3576.
[6] 林茜,黄琦.疏肝解郁活血化瘀法治疗口腔扁平苔藓30例临床观察[J].微创医学,2005,24(4):566-567.
[7] Yu G, Zhang Y, Ren W, et al. Network pharmacology-based identification of key pharmacological pathways of Yin-Huang-Qing-Fei capsule acting on chronic bronchitis[J]. Int J Chron Obstruct Pulmon Dis, 2016, 12:85-94.
[8] Tang Y, Zhang Y, Li L, et al. Kunxian capsule for rheumatoid arthritis: Inhibition of inflammatory network and reducing adverse reactions through drug matching[J]. Front Pharmacol, 2020, 11:485.
[9] Toro-Domínguez D, Martorell-Marugán J, López-Domínguez R, et al. ImaGEO: integrative gene expression meta-analysis from GEO database [J]. Bioinformatics, 2019, 35(5):880-882.
[10] Ujiie D, Okayama H, Saito K, et al. KRT17 as a prognostic biomarker for stage Ⅱ colorectal cancer [J]. Carcinogenesis, 2020, 41(5):591-599.
[11] Chiang CH, Wu CC, Lee LY, et al. Proteomics analysis reveals involvement of Krt17 in areca nut-induced oral carcinogenesis [J]. J Proteome Res, 2016, 15(9):2981-2997.
[12] Yang L, Zhang S, Wang G. Keratin 17 in disease pathogenesis:from cancer to dermatoses [J]. J Pathol, 2019, 247(2):158-165.
[13] 张平,朱瑜超,陈柳青,等.晚期角质化包膜蛋白(LCE)1B与LCE3D在银屑病皮损中的表达及意义[J].临床皮肤科杂志,2011,40(11):666-668.
[14] Ushakov RE, Skvortsova EV, Vitte MA, et al. Chondrogenic differentiation followed IGFBP3 loss in human endometrial mesenchymal stem cells [J]. Biochem Biophys Res Commun, 2020, 531(2):133-139.
[15] González-Moles Má, Warnakulasuriya S, González-Ruiz I, et al. Dysplasia in oral lichen planus: relevance, controversies and challenges. A position paper [J]. Med Oral Patol Oral Cir Bucal, 2021, 26(4):e541-e548.
[16] Carrozzo M, Uboldi CM, Dametto E, et al. Tumor necrosis factor-alpha and interferongamma polymorphisms contribute to susceptibility to oral lichen planus [J]. J Invest Dermatol, 2004, 122(1):87-94.
[17] Lukaszewska-Kuska M, Ślebioda Z, Dorocka-Bobkowska B. The effectiveness of topical forms of dexamethasone in the treatment of oral lichen planus-A systematic review [J]. Oral Dis, 2021.
[18] Gupta S, Jawanda MK. Oral lichen planus: An update on etiology, pathogenesis, clinical presentation, diagnosis and management [J]. Indian J Dermatol, 2015, 60(3):222-229.
[19] Nagpal M, Sood S. Role of curcumin in systemic and oral health: An overview [J]. J Nat Sci Biol Med, 2013, 4(1):3-7.
[20] 吴以岭.《金匮要略》活血化瘀方药研讨[J].南京中医药大学学报,1982,4:30-35.
[21] 杨淑琴,蔡剑,杨晓霞.活血化瘀方治疗口腔糜烂型扁平苔藓的临床观察[J].口腔医学,2014,34(4):288-290.
[22] Pei F, Wang HS, Chen Z, et al. Autophagy regulates odontoblast differentiation by suppressing NF-κB activation in an inflammatory environment [J]. Cell Death Dis, 2016, 7(3):e2122.
[23] Devi KP, Malar DS, Nabavi SF, et al. Kaempferol and inflammation: From chemistry to medicine [J]. Pharmacol Res, 2015, 99:1-10.