Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.

doi:10.13701/j.cnki.kqyxyj.2017.04.023

Abstract

Abstract: Objective: To explore the effect of A-type potassium channels on the proliferation of Tca8113 cells. Methods: The whole cell patch clamp technique was used to record and analyze the inhibitory effect of 4-AP on A-type K⁺ current in Tca8113 cells, and MTT tests were used to test the inhibitory effect of 4-AP on cell proliferation in vitro. Results: Under the concentration of 2 mmol/L 4-AP, the peak of A type K⁺ current was decreased from (267.04±13.84)pA to (124.81 ±5.24)pA. The different concentrations of 4-AP could inhibit the proliferation of Tca8113 cells, and the magnitude of inhibitive effect was concentrations and reaction time dependent. Conclusion: The A-type of K⁺ channels play a role in the process of Tca8113 cell proliferation, and the blocker of A-type K⁺ channel can inhibit the proliferation of Tca8113 cells.

Key words: Tongue neoplasms , Squamous cell 4-amino pyridine, Potassium channels , Voltage-gated, Cell proliferation

CLC Number:

R739.8

ZHANG Xiao-yan, CHENG Jun, XU Hua-li, MAO Liang, NIE Min-hai.. Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.[J]. Journal of Oral Science Research, 2017, 33(4): 440-443.

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