Effect of Chronic Fluorosis Environment on Expression of VEGF and eNOS in Periodontal Tissue During Orthodontic Tooth Movement in Rats

doi:10.13701/j.cnki.kqyxyj.2024.08.012

Abstract

Abstract: Objective: To observe the expression changes of periodontal tissue, vascular endothelial growth factor (VEGF), and endothelial nitric oxide synthase (eNOS) during tooth movement in SD rats with chronic fluorosis, and to preliminarily explore the possible effects of chronic fluorosis on the angiogenesis of periodontal tissue in orthodontic tooth movement rats. Methods: One hundred and twenty 3-week-old SPF grade SD rats weighing (60±5) g were selected and randomly divided into blank group (C), fluoride group (F), orthodontic group (O), and fluoride orthodontic group (FO), with 15 males and 15 females in each group. According to the duration of orthodontic force, they were divided into 0, 3, 7, 14, and 28 day groups. Each group of rats was euthanized at corresponding time points, and HE staining was used to observe the vascular status of periodontal tissue. Immunohistochemical staining was used to observe the expression of VEGF and eNOS proteins in rat periodontal tissue. Results: The models of chronic fluorosis rats and orthodontic tooth movement rats were successfully established. The arrangement of periodontal fibers in Group O was more disordered than that in Group C, with an increase in the number of periodontal blood vessels and irregular lumens. The distribution of angiogenesis in the FO group was less prominent than that in the O group. There was no significant gender difference in the expression of VEGF and eNOS between male and female rats in each group. There was no significant statistical difference in the expression of VEGF and eNOS on the tension side compared to the pressure side in the O and FO groups. The average protein expression levels of VEGF and eNOS in the FO group were higher than those in the F group, but lower than those in the O group (P<0.05). The expression of VEGF and eNOS proteins in the O and FO groups showed an increasing trend followed by a decreasing trend over time, with a peak on the third day. Conclusion: Chronic fluorosis can inhibit the expression of VEGF and eNOS in the periodontal tissue of rats during tooth movement.

Key words: chronic fluorosis, orthodontic tooth movement, periodontal tissue, VEGF, ENOS

LIU Xingyun, LI Zhongwei, JIA Ying, DING Xue. Effect of Chronic Fluorosis Environment on Expression of VEGF and eNOS in Periodontal Tissue During Orthodontic Tooth Movement in Rats[J]. Journal of Oral Science Research, 2024, 40(8): 727-734.

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