Effects of Biomimetic Nanotubes on Osteoblast Bone Gene Expression.

Abstract

Abstract: Objective: To investigate preparation parameters of TiO₂ nanotube layer by biomimetic modification, and to evaluate the osteoblast bone gene expression in vitro. Methods: TiO₂ nanotube layer was grown using anodization method. TiO₂ nanotube layer was modified by biomimetic preparation. SEM and EDS experiment methods were employed to characterize the samples. Osteoblasts were cultivated on the surfaces, and the growth of osteoblasts was then evaluated and analyzed. Results: The Ti implant with nano-Ca-P was acquired by SEM and EDS characterization. The result of real-time PCR showed that the biomimetic nanotubes could advance the ALP and OCN gene expression(P<0.05). Conclusion: The nanotubes with nano-Ca-P can express higher bone gene level comprared with nanotubes.

Key words: Nanotubes , Biomimetic, Osteoblast, Bone gene

CLC Number:

R783.1

YU Wei-qiang, XU Ling, ZHANG Fu-qiang. Effects of Biomimetic Nanotubes on Osteoblast Bone Gene Expression.[J]. Journal of Oral Science Research, 2015, 31(4): 320-323.

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