Effect of Integrin Alpha 6 on Proliferation and Odontoblast Differentiation of Human Dental Pulp Stem Cells

doi:10.13701/j.cnki.kqyxyj.2020.12.012

Abstract

Abstract: Objective: To investigate the effect of integrin alpha 6 on proliferation and odontoblast differentiation of human dental pulp stem cells (hDPSCs). Methods: HDPSCs were isolated and cultured by enzyme digestion in vitro, and the integrin α6 silencing lentivirus was constructed to infect hDPSCs. The hDPSCs were divided into negative control group (shMock group) and integrin α6 silencing group (shITGA6 group). 3-(4,5-dimethylthithioazole-2)-2,5-diphenyltetrazole bromide (MTT) and Ki-67 immunofluorescence staining were used to detect the effect of integrin α6 on the proliferation of hDPSCs. Alkaline phosphatase (ALP) staining and Western blot were used to detect the expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein-1(DMP-1), and alizarin red staining was used to detect the effect of integrin α6 on the odontoblast differentiation and mineralization of hDPSCs. Results: MTT results showed that the proliferation capacity of hDPSCs in shITGA6 group was significantly reduced (P<0.01). The immunofluorescence staining of Ki-67 showed that the positive cell rate of Ki-67 in shITGA6 group was significantly lower than that in shMock group (P<0.001). Alkaline phosphatase staining showed that ALP staining in shITGA6 group was obviously deeper. Western blot showed that the expression of DSPP and DMP-1 in shITGA6 group was significantly up-regulated than that of shMock group (P<0.05). Alizarin red staining showed that the mineralized nodules increased significantly in shITGA6 group. Conclusion: Integrin α6 can promote the proliferation of hDPSCs and inhibit the odontoblast differentiation of hDPSCs.

Key words: Integrin α6, human dental pulp stem cell, proliferation, odontoblast differentiation

YIN Xiaowei, ZHANG Shuang, DENG Haotian, HE Lina, LI Yanping, ZHANG Weiwei, PAN Shuang, NIU Yumei. Effect of Integrin Alpha 6 on Proliferation and Odontoblast Differentiation of Human Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2020, 36(12): 1132-1136.

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