Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts

doi:10.13701/j.cnki.kqyxyj.2020.05.021

Abstract

Abstract: Objective: To verify the expression of TRPM4 in buccal mucosal fibroblasts and explore its effects on migration and proliferation of buccal fibroblasts. Methods: Human buccal mucosa fibroblasts were isolated and cultured in vitro, and the expression of TRPM4 in fibroblasts was evaluated by reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence. Whole cell patch clamp were performed to record the whole cell currents of TRPM4 channels. The effects of 9-phenanthrene or TRPM4 specific siRNA on the proliferation and migration of fibroblasts were estimated by MTT assay and cell scratch test, respectively. Results: TRPM4 was functional expressed in human buccal mucosa fibroblasts. Suppression the function of TRPM7 channel by 9-phenanthrene or specific siRNA inhibited the proliferation and migration capability of the cells. Conclusion: TRPM4 channels is involved in the regulation of proliferation and migration in human buccal mucosa fibroblasts.

Key words: buccal mucosal fibroblast, TRPM4, cell proliferation, cell migration

WANG Xueteng, NIE Minhai, ZUO Dongchuan, DENG Hao, ZENG Jin. Effect of TRPM4 Channel on Proliferation and Migration of Human Buccal Mucosal Fibroblasts[J]. Journal of Oral Science Research, 2020, 36(5): 490-495.

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