Changes of Tfh Cells in SD Rats Immunized with Periodontitis DNA  Vaccine pVAX1-HA2-FimA-(IL-15) through Nasal Mucosa

doi:10.13701/j.cnki.kqyxyj.2020.04.011

Abstract

Abstract: Objective: To investigate the changes of Tfh cells in SD rats immunized with pVAX1-HA2-FimA-(IL- 15) eriodontitis gene vaccine, and to investigate the role of Tfh cells in mucosal immune response to produce specific SIgA antibody. Methods: SD rats were randomly divided into three groups. Group A was nasal saline, group B was nasal drop vector (pVAX1), and group C was nasal drop periodontitis vaccine pVAX1-HA2-FimA-(IL- 15). ELISA was used to detect the level of specific SIgA antibody in rat saliva, immunofluorescence technique was used to detect the localization of Tfh cells in nasal mucosa, and flow cytometry was used to detect Tfh cells in nasal mucosa and CD4+CXCR5⁺ICOS⁺Tfh Expression of cells. Results: The expression level of specific SIgA antibody in saliva of group C was always higher than that of group A and group B (P<0.05). The fusion of FITC(CD4) and Aleax flour 594(CXCR5) in group C showed yellow fluorescence, and the double immunofluorescence staining results of group A and group B showed no double positive fluorescence signal. The expression of CD4⁺CXCR5⁺Tfh and CD4⁺CXCR5⁺ICOS⁺ Tfh in nasal mucosa of group C rats was higher than those of group A and group B (P<0.05). The specific SIgA in rat saliva and rat nasal mucosa CD4 were found. There were correlations between CD4⁺CXCR5⁺Tfh and CD4⁺CXCR5⁺ICOS⁺Tfh cells. Conclusion: pVAX1-HA2-FimA-(IL- 15), a genetic vaccine for periodontitis, can activate the expression of Tfh cells in nasal mucosa after immunizing rats with specific SIgA antibody, and the expression level is positively correlated with the level of specific SIgA antibody, suggesting that Tfh cells may play an important role in promoting the production of specific SIgA antibody in the body.

Key words: gene vaccine for periodontitis, porphyromonas gingival, mucosal immunity, follicular helper T lymphocytes, secretory immunoglobulin A

TIAN Yuan, CHEN Jing, ZENG Fengjiao, YU Hang, LIU Xia, LIU Jianguo, BAI Guohui. Changes of Tfh Cells in SD Rats Immunized with Periodontitis DNA Vaccine pVAX1-HA2-FimA-(IL-15) through Nasal Mucosa[J]. Journal of Oral Science Research, 2020, 36(4): 350-354.

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Changes of Tfh Cells in SD Rats Immunized with Periodontitis DNA Vaccine pVAX1-HA2-FimA-(IL-15) through Nasal Mucosa

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