Study of Simulated Microgravity on Cytoskeleton and Migration Ability of hDPSCs.

Abstract

Abstract: Objective: To investigate the effect of RhoA/ROCK signaling pathway on cytoskeleton and migration ability of human dental pulp stem cells (hDPSCs) in simulated microgravity (SMG). Methods: hDPSCs were seeded on PLGA scaffolds, and were cultured both on the conditions of conventional environment and SMG for 72 hours. The expression levels of LARG and GAPDH mRNA were measured using the real-time quantitative PCR method. Pull-down assay and western-blot were conducted to detect the RhoA-GTP,RhoA,LIMK-P expression levels. Results: The gene expression of LARG, a member of the Rho guanine nucleotide exchange factor (GEF) family, is observed to be downregulated under SMG(P<0.05);The RhoA-GTP,RhoA,LIMK-P protein expression levels are downregulated under microgravity(P<0.05). Conclusion: Simulated microgravity can inhibit RhoA/ROCK signaling pathway to change human dental pulp stem cells (hDPSCs) cytoskeleton structure and enhance the adhesion ability, reduce the ability to migrate.

Key words: Human dental pulp stem cells, Simulated microgravity , RhoA Migration

CLC Number:

R780.11

WU Xiang-zhou, PAN Shuang, LI Yan-ping, et al. Study of Simulated Microgravity on Cytoskeleton and Migration Ability of hDPSCs.[J]. Journal of Oral Science Research, 2015, 31(2): 137-139.

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