Activation of Triggering Receptor Expressed on Myeloid Cells by Lipopolysacchride from Porphyromonas Gingivalis.

Abstract

Abstract: Objective: To investigate whether porphyromonas gingivalis lipopolysacchride(Pg-LPS) can activate triggering receptor expressed on myeloid cells to stimulate inflammatory response. Methods: Mice peritoneal macrophages were separated and cultured, then treated with Pg-LPS. Quantitative real time PCR was utilized to detect mRNA transcription of TLR-2, TLR-4, TREM-1 and TREM-2, and flow cytometry was used to quantify protein expression,and tumor necrosis factor-α(TNF-α) and macrophage inflammatory protein-1α(MIP-lα) in supernatants were detected by enzyme linked absorbent analysis (ELISA). Results: 2 h after Pg-LPS incubation, TLR-2 and TREM-1 mRNA transcription was up-regulated; 24 h after Pg-LPS treatment, increased TLR-2 and TREM-1 protein expression was detected; Pg-LPS did not significantly activate TLR-4 and TREM-2;Pg-LPS triggered TNF-α and MIP-lα release into cell culture. LP-17 polypeptide, an inhibitor of TREM-1, inhibited Pg-LPS stimulated cytokine production. Conclusion: TLR-2 and TREM-1 were involved in innate immune response to Pg-LPS.

Key words: Porphyromonas gingivalis , Polyliposacchride, Triggering receptor expressed on myeloid cells

CLC Number:

R780.2

HONG Fei-fei, LEI Lang, PAN Sheng-bo, et al.. Activation of Triggering Receptor Expressed on Myeloid Cells by Lipopolysacchride from Porphyromonas Gingivalis.[J]. Journal of Oral Science Research, 2015, 31(3): 213-216.

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